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Gene expression and promoter methylation of porcine uncoupling protein 3 gene
- Source :
- Asian-Australasian Journal of Animal Sciences, Vol 32, Iss 2, Pp 170-175 (2019), Asian-Australasian Journal of Animal Sciences
- Publication Year :
- 2019
- Publisher :
- Asian-Australasian Association of Animal Production Societies, 2019.
-
Abstract
- Objective Uncoupling protein 3 gene (UCP3) is a candidate gene associated with the meat quality of pigs. The aim of this study was to explore the regulation mechanism of UCP3 expression and provide a theoretical basis for the research of the function of porcine UCP3 gene in meat quality. Methods Bisulfite sequencing polymerase chain reaction (PCR) and quantitative real-time PCR (Q-PCR) were used to analyze the methylation of UCP3 5'-flanking region and UCP3 mRNA expression in the adipose tissue or skeletal muscle of three pig breeds at different ages (1, 90, 210-day-old Putian Black pig; 90-day-old Duroc; and 90-day-old Dupu). Results Results showed that two cytosine-guanine dinucleotide (CpG) islands are present in the promoter region of porcine UCP3 gene. The second CpG island located in the core promoter region contained 9 CpG sites. The methylation level of CpG island 2 was lower in the adipose tissue and skeletal muscle of 90-day-old Putian Black pigs compared with 1-day-old and 210-day-old Putian Black pigs, and the difference also existed in the skeletal muscle among the three 90-day-old pig breeds. Furthermore, the obvious changing difference of UCP3 mRNA expression was observed in the skeletal muscle of different groups. However, the difference of methylation status and expression level of UCP3 gene was not significant in the adipose tissue. Conclusion Our data indicate that UCP3 mRNA expression level was associated with the methylation status of UCP3 promoter in the skeletal muscle of pigs.
- Subjects :
- Bisulfite sequencing
lcsh:Animal biochemistry
Adipose tissue
Expression
Biology
Methylation
Article
Gene expression
medicine
lcsh:QP501-801
UCP3
lcsh:SF1-1100
Pig
0402 animal and dairy science
Skeletal muscle
Promoter
04 agricultural and veterinary sciences
Animal Breeding and Genetics
040201 dairy & animal science
Molecular biology
medicine.anatomical_structure
CpG site
Meat Quality
Animal Science and Zoology
lcsh:Animal culture
Uncoupling Protein 3 (UCP3) Gene
Food Science
Uncoupling Protein 3 () Gene
Subjects
Details
- Language :
- English
- ISSN :
- 19765517 and 10112367
- Volume :
- 32
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Asian-Australasian Journal of Animal Sciences
- Accession number :
- edsair.doi.dedup.....92c174d53f0995d7c113fa5a242cef34