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Simultaneously Quantitative Analysis of Naringin and Its Major Human Gut Microbial Metabolites Naringenin and 3-(4′-Hydroxyphenyl) Propanoic Acid via Stable Isotope Deuterium-Labeling Coupled with RRLC-MS/MS Method
- Source :
- Molecules, Volume 24, Issue 23
- Publication Year :
- 2019
- Publisher :
- MDPI AG, 2019.
-
Abstract
- Widespread in citrus fruits, naringin, a natural 2,3-dihydroflavonoid, is of particular interest to scientists and has a broad range of beneficial bioactivities to health. Orally administered naringin remains in the gut tract for a relatively long time because of its low bioavailability. Under the metabolism mediated by human gut microbiota, naringin could be an active precursor for derived metabolites to play important physiological roles. However, naringin and its metabolites are hard to accurately quantify due to severe endogenic interference. In this study, an analytical rapid resolution liquid chromatography tandem mass spectrometry (RRLC-MS/MS) method coupled with stable isotope deuterium-labeling is developed and validated to simultaneously quantify naringin as well as its major human gut microbial metabolites naringenin and 3-(4&prime<br />hydroxyphenyl) propanoic acid. By eliminating the matrix interferences, this strategy not only confirms naringenin and 3-(4&prime<br />hydroxyphenyl) propanoic acid as the predominant metabolites which contribute to the pharmacological effects of naringin but also provides a suitable choice for other flavonoid pharmacokinetics study.
- Subjects :
- Naringenin
Citrus
microbial metabolites
stable isotope deuterium-labeling
Flavonoid
Pharmaceutical Science
01 natural sciences
Article
Analytical Chemistry
03 medical and health sciences
chemistry.chemical_compound
Pharmacokinetics
Tandem Mass Spectrometry
Liquid chromatography–mass spectrometry
gut microorganism
Drug Discovery
RRLC-MS/MS
Humans
Physical and Theoretical Chemistry
Naringin
Chromatography, High Pressure Liquid
030304 developmental biology
Flavonoids
chemistry.chemical_classification
0303 health sciences
Chromatography
naringin
010401 analytical chemistry
Organic Chemistry
food and beverages
Deuterium
Gastrointestinal Microbiome
0104 chemical sciences
Bioavailability
Propanoic acid
chemistry
Chemistry (miscellaneous)
Isotope Labeling
Flavanones
Metabolome
Molecular Medicine
Propionates
Quantitative analysis (chemistry)
Subjects
Details
- ISSN :
- 14203049
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....9299801abc45fc36479a2334cd3ec1dd