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Isolation, expression, and functional analysis of developmentally regulated plasma membrane polypeptide 1 (DREPP1) in Sporobolus virginicus grown under alkali salt stress
- Source :
- Protoplasma. 255(5)
- Publication Year :
- 2017
-
Abstract
- The plant specific DREPP proteins have been shown to bind Ca2+ and regulate the N-myristoylation signaling and microtubule polymerization in Arabidopsis thaliana. The information about DREPP proteins in other plants is, however, scarce. In the present study, we isolated the DREPP gene from a halophytic grass, Sporobolus virginicus, and tested whether the gene was involved in alkaline salt stress responses. The SvDREPP1 was cloned from S. virginicus by RACE methods. The isolated gene showed high homology to DREPP homologs from C4 grasses, Setaria italica, and Panicum hallii as well as rice (OsDREPP1). The encoded protein contained 202 amino acid residues. It was expressed in E. coli, and its biochemical properties were studied. It was observed that SvDREPP1 was not only Ca2+-binding protein, but also bind to calmodulin and microtubules. The SvDREPP1 mRNA expression in plants grown under alkaline salt stress was upregulated by 3.5 times over the control in leaf tissues after 48-h treatment, whereas it was increased for 6.0 times in the root tissues at 36 h. The data suggests the importance of SvDREPP1 in regulating alkali salt stress responses in the leaf tissues.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Setaria
Calmodulin
Plant Science
Sodium Chloride
Poaceae
01 natural sciences
Plant Roots
Microtubule polymerization
03 medical and health sciences
Microtubule
Gene Expression Regulation, Plant
Halophyte
Arabidopsis thaliana
RNA, Messenger
Gene
Plant Proteins
biology
Chemistry
Calcium-Binding Proteins
food and beverages
Salt-Tolerant Plants
Cell Biology
General Medicine
biology.organism_classification
Plant Leaves
Proton-Translocating ATPases
030104 developmental biology
Biochemistry
biology.protein
Sporobolus virginicus
010606 plant biology & botany
Subjects
Details
- ISSN :
- 16156102
- Volume :
- 255
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Protoplasma
- Accession number :
- edsair.doi.dedup.....92955c53011ebdf2dda9953fbfc6458e