Back to Search Start Over

Intratumoral T cell subset ratios and Fas ligand expression on brain tumor endothelium

Authors :
Ken Samoto
John S. Yu
Keith L. Black
Moneeb Ehtesham
Paul K. Lee
Christopher J. Wheeler
Source :
Journal of Neuro-oncology. 64:55-61
Publication Year :
2003
Publisher :
Springer Science and Business Media LLC, 2003.

Abstract

Introduction: T cell presence in TIL, and the ratio of CD8+ and CD4+ T cell subsets in particular, can correlate with tumor prognosis in some tumors, although the significance of such infiltration into glioma is controversial. However, gliomas represent a lower extreme in their extent of T cell infiltration, and are thus useful in assessing factors that can decrease T cell presence within tumor tissue. Fas ligand, a pro-apoptotic cell surface protein, may play a key role in reduction of T cells in tumor tissue. Objective: To assess the level of FasL expression on brain tumor endothelium and to correlate this with relative levels of CD4+ and CD8+ T cell subsets in TIL from brain tumors. Methods: CD3+, CD4+, and CD8+ cells were quantified in fresh TIL by flow cytometry. Paraffin embedded sections of tumors, including meningiomas and gliomas as well as extracranial malignancies, underwent immunohistochemical staining for FasL and Von-Willebrand’s factor (Factor VIII) to determine expression levels of endothelial FasL. Results: FasL expression was high in aggressive intracranial malignancies compared to more indolent neoplasms, and correlated inversely with CD8+/CD4+ TIL ratios in all tumor classes combined (ANOVA,p

Details

ISSN :
15737373 and 0167594X
Volume :
64
Database :
OpenAIRE
Journal :
Journal of Neuro-oncology
Accession number :
edsair.doi.dedup.....928fc12fcf69f0892700f1796acd505e
Full Text :
https://doi.org/10.1007/bf02700020