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Specific human antibody responses to Aedes aegypti and Aedes polynesiensis saliva: A new epidemiological tool to assess human exposure to disease vectors in the Pacific
- Source :
- PLoS Neglected Tropical Diseases, Vol 12, Iss 7, p e0006660 (2018), PLoS Neglected Tropical Diseases, PLoS Neglected Tropical Diseases, 2018, ⟨10.1371/journal.pntd.0006660⟩, PLoS Neglected Tropical Diseases, Public Library of Science, 2018, ⟨10.1371/journal.pntd.0006660⟩
- Publication Year :
- 2018
- Publisher :
- Public Library of Science (PLoS), 2018.
-
Abstract
- Background Aedes mosquitoes severely affect the health and wellbeing of human populations by transmitting infectious diseases. In French Polynesia, Aedes aegypti is the main vector of dengue, chikungunya and Zika, and Aedes polynesiensis the primary vector of Bancroftian filariasis and a secondary vector of arboviruses. Tools for assessing the risk of disease transmission or for measuring the efficacy of vector control programmes are scarce. A promising approach to quantify the human-vector contact relies on the detection and the quantification of antibodies directed against mosquito salivary proteins. Methodology/Principal findings An ELISA test was developed to detect and quantify the presence of immunoglobulin G (IgG) directed against proteins from salivary gland extracts (SGE) of Ae. aegypti and Ae. polynesiensis in human populations exposed to either species, through a cross-sectional study. In Tahiti and Moorea islands where Ae. aegypti and Ae. polynesiensis are present, the test revealed that 98% and 68% of individuals have developed IgG directed against Ae. aegypti and Ae. polynesiensis SGE, respectively. By comparison, ELISA tests conducted on a cohort of people from metropolitan France, not exposed to these Aedes mosquitoes, indicated that 97% of individuals had no IgG directed against SGE of either mosquito species. The analysis of additional cohorts representing different entomological Aedes contexts showed no ELISA IgG cross-reactivity between Ae. aegypti and Ae. polynesiensis SGE. Conclusions/Significance The IgG response to salivary gland extracts seems to be a valid and specific biomarker of human exposure to the bites of Ae. aegypti and Ae. polynesiensis. This new immuno-epidemiological tool will enhance our understanding of people exposure to mosquito bites, facilitate the identification of areas where disease transmission risk is high and permit to evaluate the efficacy of novel vector control strategies in Pacific islands and other tropical settings.<br />Author summary In Pacific islands like in most tropical regions, Aedes mosquitoes affect the health of human populations by transmitting diseases like dengue, chikungunya, Zika and filariasis. The biting nuisance of Aedes mosquitoes also impacts local tourism, affecting the sustainability of island economies. Mosquito saliva is injected during the biting process, and the response triggered by the human immune system to proteins contained in mosquito saliva was shown to be a relevant biomarker of exposure to mosquito bites. Using this approach, we have developed an immuno-epidemiological tool to investigate the exposure of people to the bites of Aedes aegypti and Aedes polynesiensis, two significant mosquito vectors of infectious diseases in French Polynesia and other island countries and territories in the Pacific. This novel tool proved specific and reliable. It will improve the assessment of disease transmission risk and be useful for measuring the efficacy of both conventional and innovative vector control strategies.
- Subjects :
- Male
Saliva
Topography
Physiology
French Polynesia
Social Sciences
Disease Vectors
medicine.disease_cause
Mosquitoes
Immunoglobulin G
Geographical locations
Dengue fever
Cohort Studies
Governments
0302 clinical medicine
Aedes
Medicine and Health Sciences
030212 general & internal medicine
Chikungunya
Enzyme-Linked Immunoassays
Child
ComputingMilieux_MISCELLANEOUS
Islands
biology
lcsh:Public aspects of medicine
Eukaryota
Middle Aged
Aedes polynesiensis
3. Good health
Body Fluids
Insects
Infectious Diseases
Military Personnel
Insect Proteins
Female
Anatomy
Research Article
Adult
lcsh:Arctic medicine. Tropical medicine
Adolescent
Arthropoda
Infectious Disease Control
lcsh:RC955-962
Political Science
030231 tropical medicine
Oceania
Enzyme-Linked Immunosorbent Assay
Aedes aegypti
Mosquito Vectors
Aedes Aegypti
Pacific Islands
Research and Analysis Methods
Polynesia
03 medical and health sciences
Young Adult
medicine
Animals
Humans
Salivary Proteins and Peptides
Immunoassays
Landforms
Public Health, Environmental and Occupational Health
Organisms
Insect Bites and Stings
Biology and Life Sciences
Geomorphology
lcsh:RA1-1270
biology.organism_classification
medicine.disease
Virology
Invertebrates
Insect Vectors
Species Interactions
Cross-Sectional Studies
Vector (epidemiology)
Antibody Formation
biology.protein
Earth Sciences
Immunologic Techniques
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
People and places
Armed Forces
Subjects
Details
- Language :
- English
- ISSN :
- 19352735 and 19352727
- Volume :
- 12
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- PLoS Neglected Tropical Diseases
- Accession number :
- edsair.doi.dedup.....92817c80af3aabac64ec5fb870d60378