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A Bivalent Vaccine Based on a PB2-Knockout Influenza Virus Protects Mice From Secondary Pneumococcal Pneumonia
- Source :
- Journal of Infectious Diseases. 212:1939-1948
- Publication Year :
- 2015
- Publisher :
- Oxford University Press (OUP), 2015.
-
Abstract
- Background Secondary bacterial infections after influenza can be a serious problem, especially in young children and the elderly, yet the efficacy of current vaccines is limited. Earlier work demonstrated that a replication-incompetent PB2-knockout (PB2-KO) influenza virus possessing a foreign gene in the coding region of its PB2 segment can serve as a platform for a bivalent vaccine. Methods In the current study, we generated the PB2-KO virus expressing pneumococcal surface protein A (PspA), PB2-KO-PspA virus, the replication of which is restricted to PB2-expressing cells. We then examined the protective efficacy of intranasal immunization with this virus as a bivalent vaccine in a mouse model. Results High levels of influenza virus-specific and PspA-specific antibodies were induced in the serum and airways of immunized mice. The intranasally immunized mice were protected from lethal doses of influenza virus or Streptococcus pneumoniae. These mice were also completely protected from secondary pneumococcal pneumonia after influenza virus infection. Conclusions These findings indicate that our recombinant influenza virus serves as a novel and powerful bivalent vaccine against primary and secondary pneumococcal pneumonia as well as influenza.
- Subjects :
- viruses
medicine.disease_cause
Bivalent (genetics)
Virus
law.invention
Pneumococcal Vaccines
Gene Knockout Techniques
Mice
Viral Proteins
Major Articles and Brief Reports
Bacterial Proteins
Orthomyxoviridae Infections
law
Streptococcus pneumoniae
Animals
Immunology and Allergy
Medicine
Gene
Administration, Intranasal
Vaccines, Synthetic
biology
Coinfection
business.industry
food and beverages
virus diseases
Pneumonia, Pneumococcal
biochemical phenomena, metabolism, and nutrition
Orthomyxoviridae
medicine.disease
Virology
respiratory tract diseases
Disease Models, Animal
Infectious Diseases
Influenza Vaccines
Immunology
Pneumococcal pneumonia
Recombinant DNA
biology.protein
Nasal administration
Antibody
business
Subjects
Details
- ISSN :
- 15376613 and 00221899
- Volume :
- 212
- Database :
- OpenAIRE
- Journal :
- Journal of Infectious Diseases
- Accession number :
- edsair.doi.dedup.....927afd3cd6aff4689380cdd248ed6d73