Mapping Bone Marrow Cell Response from Senile Female Rats on Ca-P-Doped Titanium Coating

Made available in DSpace on 2022-04-28T19:50:10Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-02-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Chemical and topographical surface modifications on dental implants aim to increase the bone surface contact area of the implant and improve osseointegration. This study analyzed the cellular response of undifferentiated mesenchymal stem cells (MSC), derived from senile rats’ femoral bone marrow, when cultured on a bioactive coating (by plasma electrolytic oxidation, PEO, with Ca2+ and P5+ ions), a sandblasting followed by acid-etching (SLA) surface, and a machined surface (MSU). A total of 102 Ti-6Al-4V discs were divided into three groups (n = 34). The surface chemistry was analyzed by energy dispersive spectroscopy (EDS). Cell viability assay, gene expression of osteoblastic markers, and mineralized matrix formation were investigated. The cell growth and viability results were higher for PEO vs. MSU surface (p = 0.001). An increase in cell proliferation from 3 to 7 days (p < 0.05) and from 7 to 10 days (p < 0.05) was noted for PEO and SLA surfaces. Gene expression for OSX, ALP, BSP, and OPN showed a statistical significance (p = 0.001) among groups. In addition, the PEO surface showed a higher mineralized matrix bone formation (p = 0.003). In conclusion, MSC from senile female rats cultured on SLA and PEO surfaces showed similar cellular responses and should be considered for future clinical investigations. Department of Diagnosis and Surgery Division of Oral and Maxillofacial Surgery and Implantology School of Dentistry São Paulo State University—UNESP Dental Prosthesis Department of Dental Materials and Prosthodontics School of Dentistry São Paulo State University—UNESP Bone Res Lab School of Dentistry of Ribeirao Preto University of São Paulo (USP) Department of Periodontology and Oral Implantology Dental Research Division University of Guarulhos (UnG) Department of Prosthodontics and Periodontology Piracicaba Dental School University of Campinas (UNICAMP) Department of Restorative Dentistry College of Dentistry University of Illinois at Chicago Department of Diagnosis and Surgery Division of Oral and Maxillofacial Surgery and Implantology School of Dentistry São Paulo State University—UNESP Dental Prosthesis Department of Dental Materials and Prosthodontics School of Dentistry São Paulo State University—UNESP FAPESP: 2016/20297-6