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Mannan oligosaccharide increases the growth performance, immunity and resistance capability against Vibro Parahemolyticus in juvenile abalone Haliotis discus hannai Ino
- Source :
- Fish & Shellfish Immunology. 94:654-660
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- This trial was conducted to investigate the effect of mannose oligosaccharides (MOS) on the growth performance, antioxidation, immunity and disease resistance of Vibro Parahemolyticus in juvenile abalone Haliotis discus hannai Ino. Four formulated diets were produced to contain 0.00 g/kg, 0.40 g/kg, 0.80 g/kg and 1.60 g/kg Actigen®, with functional ingredients of MOS, respectively. Accordingly, the experimental diets were named as A0, A4, A8 and A16. After 120-days feeding trial, the best growth performance was observed in A8 group (P 0.05) and there was no significant difference in A0, A4 and A16 groups. With the increase of dietary MOS, the activity of the total antioxidant capacity in hepatopancreas is increasingly elevated (P 0.05) while no significant difference was observed on activity of glutathione S-transferase (P 0.05). The activities of superoxide dismutase and glutathione peroxidase were firstly increased and then decreased, with the highest values in A8 group (P 0.05). Immune-related parameters were significantly affected by dietary MOS inclusion. Specifically, the activities of alkaline phosphatase and acid phosphatase in hepatopancreas and serum of abalone fed diets containing MOS were significantly higher than those of control A0 group (P 0.05). Moreover, the highest values of both enzymes were observed in hepatopancreas of A8 group but in serum of A16 group, respectively. The lysozyme activities in hepatopancreas and serum of A4 group were significantly higher than those of other groups (P 0.05) and there was no significant difference in A0, A8 and A16 groups (P 0.05). The activities of cytophagy and respiratory burst in serum of abalone were not significantly affected by dietary MOS content (P 0.05). The mRNA levels of focal adhesion kinase and integrin-linked kinase were gradually elevated with the increase of dietary MOS, with the highest value recorded in A16 group (P 0.05). The gene expression of caspse-3 in A8 group was dramatically higher than those of other groups (P 0.05) and there was no significant difference in A0, A4 and A16 groups (P 0.05). The mRNA level of nuclear factor-κB was not significantly affected by dietary MOS (P 0.05). During 56 h of V. Parahemolyticus challenge period, the accumulated mortality rate of abalone fed diets containing MOS were significantly lower than that of control A0 group in each time point (P 0.05). Overall, the lowest rate was happened in A8 group (P 0.05). In conclusion, MOS inclusion in diet has obviously positive effect on growth, immunity and disease resistance capability of abalone, with the optimal level of Actigen® at 0.80 g/kg in diet.
- Subjects :
- 0301 basic medicine
Abalone
Gastropoda
Longevity
Oligosaccharides
Aquatic Science
Antioxidants
Mannans
Superoxide dismutase
03 medical and health sciences
Animal science
Immunity
Haliotis discus
Animals
Environmental Chemistry
Juvenile
Mannan
chemistry.chemical_classification
Dose-Response Relationship, Drug
biology
Glutathione peroxidase
04 agricultural and veterinary sciences
General Medicine
biology.organism_classification
Animal Feed
Immunity, Innate
Diet
Respiratory burst
030104 developmental biology
chemistry
Dietary Supplements
040102 fisheries
biology.protein
0401 agriculture, forestry, and fisheries
Vibrio parahaemolyticus
Subjects
Details
- ISSN :
- 10504648
- Volume :
- 94
- Database :
- OpenAIRE
- Journal :
- Fish & Shellfish Immunology
- Accession number :
- edsair.doi.dedup.....92424cbf59465f6e313d5f741e6a807c