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Homogeneous agglutination assay based on micro-chip sheathless flow cytometry
- Publication Year :
- 2015
- Publisher :
- AIP Publishing LLC, 2015.
-
Abstract
- Homogeneous assays possess important advantages that no washing or physical separation is required, contributing to robust protocols and easy implementation which ensures potential point-of-care applications. Optimizing the detection strategy to reduce the number of reagents used and simplify the detection device is desirable. A method of homogeneous bead-agglutination assay based on micro-chip sheathless flow cytometry has been developed. The detection processes include mixing the capture-probe conjugated beads with an analyte containing sample, followed by flowing the reaction mixtures through the micro-chip sheathless flow cytometric device. The analyte concentrations were detected by counting the proportion of monomers in the reaction mixtures. Streptavidin-coated magnetic beads and biotinylated bovine serum albumin (bBSA) were used as a model system to verify the method, and detection limits of 0.15 pM and 1.5 pM for bBSA were achieved, using commercial Calibur and the developed micro-chip sheathless flow cytometric device, respectively. The setup of the micro-chip sheathless flow cytometric device is significantly simple; meanwhile, the system maintains relatively high sensitivity, which mainly benefits from the application of forward scattering to distinguish aggregates from monomers. The micro-chip sheathless flow cytometric device for bead agglutination detection provides us with a promising method for versatile immunoassays on microfluidic platforms.
- Subjects :
- Fluid Flow and Transfer Processes
Detection limit
Analyte
Materials science
Chromatography
biology
medicine.diagnostic_test
Molecular biophysics
Microfluidics
Biomedical Engineering
Analytical chemistry
Condensed Matter Physics
Chip
Flow cytometry
Agglutination (biology)
Colloid and Surface Chemistry
biology.protein
medicine
General Materials Science
Fabrication and Laboratory Methods
Bovine serum albumin
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....9237f45b7f051be3e3cbcb22c6f62d5d