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Comprehensive identification of LMW-GS genes and their protein products in a common wheat variety
- Source :
- Functional & Integrative Genomics. 16:269-279
- Publication Year :
- 2016
- Publisher :
- Springer Science and Business Media LLC, 2016.
-
Abstract
- Although it is well known that low-molecular-weight glutenin subunits (LMW-GS) from wheat affect bread and noodle processing quality, the function of specific LMW-GS proteins remains unclear. It is important to find the genes that correspond to individual LMW-GS proteins in order to understand the functions of specific proteins. The objective of this study was to link LMW-GS genes and haplotypes characterized using well known Glu-A3, Glu-B3, and Glu-D3 gene-specific primers to their protein products in a single wheat variety. A total of 36 LMW-GS genes and pseudogenes were amplified from the Korean cultivar Keumkang. These include 11 Glu-3 gene haplotypes, two from the Glu-A3 locus, two from the Glu-B3 locus, and seven from the Glu-D3 locus. To establish relationships between gene haplotypes and their protein products, a glutenin protein fraction was separated by two-dimensional gel electrophoresis (2-DGE) and 17 protein spots were analyzed by N-terminal amino acid sequencing and tandem mass spectrometry (MS/MS). LMW-GS proteins were identified that corresponded to all Glu-3 gene haplotypes except the pseudogenes. This is the first report of the comprehensive characterization of LMW-GS genes and their corresponding proteins in a single wheat cultivar. Our approach will be useful to understand the contributions of individual LMW-GS to the end-use quality of flour.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Glutens
Pseudogene
Locus (genetics)
Biology
Proteomics
01 natural sciences
03 medical and health sciences
Glutenin
Tandem Mass Spectrometry
Genetics
Electrophoresis, Gel, Two-Dimensional
Amino Acid Sequence
Common wheat
Gene
Alleles
Triticum
Gel electrophoresis
Haplotype
food and beverages
Bread
General Medicine
Molecular Weight
030104 developmental biology
Haplotypes
biology.protein
Pseudogenes
010606 plant biology & botany
Subjects
Details
- ISSN :
- 14387948 and 1438793X
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- Functional & Integrative Genomics
- Accession number :
- edsair.doi.dedup.....9235a966dcbec2b71eef6a12fbd68a43