Back to Search
Start Over
Rapid cold hardening and octopamine modulate chill tolerance in Locusta migratoria
- Publication Year :
- 2019
- Publisher :
- Elsevier, 2019.
-
Abstract
- Temperature has profound effects on the neural function and behaviour of insects. When exposed to low temperature, chill-susceptible insects enter chill coma, a reversible state of neuromuscular paralysis. Despite the popularity of studying the effects of low temperature on insects, we know little about the physiological mechanisms controlling the entry to, and recovery from, chill coma. Spreading depolarization (SD) is a phenomenon that causes a neural shutdown in the central nervous system (CNS) and it is associated with a loss of K+ homeostasis in the CNS. Here, we investigated the effects of rapid cold hardening (RCH) on chill tolerance of the migratory locust. With an implanted thermocouple in the thorax, we determined the temperature associated with a loss of responsiveness (i.e. the critical thermal minimum – CTmin) in intact male adult locusts. In parallel experiments, we recorded field potential (FP) in the metathoracic ganglion (MTG) of semi-intact preparations to determine the temperature that would induce neural shutdown. We found that SD in the CNS causes a loss of coordinated movement immediately prior to chill coma and RCH reduces the temperature that evokes neural shutdown. Additionally, we investigated a role for octopamine (OA) in the locust chill tolerance and found that OA reduces the CTmin and mimics the effects of prior stress (anoxia) in locust.
- Subjects :
- Central Nervous System
Male
Thermotolerance
030110 physiology
0301 basic medicine
Nervous system
Spreading depolarization
Physiology
Central nervous system
Epinastine
Locusta migratoria
Cold tolerance
Biochemistry
03 medical and health sciences
medicine
Animals
Homeostasis
Octopamine
Molecular Biology
Chill coma
SD
Ganglion Cysts
biology
Chemistry
fungi
Depolarization
Migratory locust
RCH
Thorax
biology.organism_classification
Cell biology
Cold Temperature
030104 developmental biology
medicine.anatomical_structure
Potassium
Octopamine (neurotransmitter)
Cold hardening
Insect
Locust
Subjects
Details
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....920a55e5d9ad9a7ecd71a0611b305244