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Reciprocal lateral hypothalamic and raphe GABAergic projections promote wakefulness
- Source :
- J Neurosci
- Publication Year :
- 2021
- Publisher :
- Society for Neuroscience, 2021.
-
Abstract
- The lateral hypothalamus (LH), together with multiple neuromodulatory systems of the brain, such as the dorsal raphe nucleus (DR), is implicated in arousal, yet interactions between these systems are just beginning to be explored. Using a combination of viral tracing, circuit mapping, electrophysiological recordings from identified neurons, and combinatorial optogenetics in mice, we show that GABAergic neurons in the LH selectively inhibit GABAergic neurons in the DR, resulting in increased firing of a substantial fraction of its neurons that ultimately promotes arousal. These DRGABAneurons are wake active and project to multiple brain areas involved in the control of arousal, including the LH, where their specific activation potently influences local network activity leading to arousal from sleep. Our results show how mutual inhibitory projections between the LH and the DR promote wakefulness and suggest a complex arousal control by intimate interactions between long-range connections and local circuit dynamics.SIGNIFICANCE STATEMENT:Multiple brain systems including the lateral hypothalamus and raphe serotonergic system are involved in the regulation of the sleep/wake cycle, yet the interaction between these systems have remained elusive. Here we show that mutual disinhibition mediated by long range inhibitory projections between these brain areas can promote wakefulness. The main importance of this work relies in revealing the interaction between a brain area involved in autonomic regulation and another in controlling higher brain functions including reward, patience, mood and sensory coding.
- Subjects :
- 0301 basic medicine
Dorsal Raphe Nucleus
Male
Lateral hypothalamus
Biology
Optogenetics
Arousal
03 medical and health sciences
Mice
0302 clinical medicine
Dorsal raphe nucleus
Neuromodulation
Neural Pathways
medicine
Animals
GABAergic Neurons
Wakefulness
610 Medicine & health
Research Articles
Raphe
General Neuroscience
030104 developmental biology
medicine.anatomical_structure
Hypothalamic Area, Lateral
GABAergic
Sleep
Neuroscience
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 02706474
- Database :
- OpenAIRE
- Journal :
- J Neurosci
- Accession number :
- edsair.doi.dedup.....9205e5ad675afabe9854cf3800199359