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Prevention of age-related memory deficit in transgenic mice by human choline acetyltransferase
- Source :
- European journal of pharmacology. 683(1-3)
- Publication Year :
- 2011
-
Abstract
- Choline acetyltransferase (ChAT, acetylCoA:choline O-acetyltransferase, EC 2.3.1.6) is the biosynthetic enzyme of neurotransmitter acetylcholine. Here we showed for the first time that transgenic mice with human ChAT kept excellent learning and memory ability during aging process. Transgenic mice were prepared through microinjection of human ChAT into mouse fertilized eggs, and PCR reaction was used to screen out the transgenic mice. The results of measurements of ChAT activity and acetylcholine level in mouse brain indicated that human ChAT gene was expressed throughout the life of the transgenic mice. The results of step-through test and water maze test suggested that learning and memory ability was improved in transgenic mice compared to that of their age-matched littermates. The results support our idea that supplement of ChAT might serve as a potential therapeutic strategy for cognitive deficit.
- Subjects :
- Genetically modified mouse
medicine.medical_specialty
Aging
Transgene
education
Mice, Transgenic
Nerve Tissue Proteins
Water maze
Choline O-Acetyltransferase
chemistry.chemical_compound
Mice
Memory
Internal medicine
mental disorders
medicine
Avoidance Learning
Choline
Animals
Humans
Neurotransmitter
Maze Learning
Microinjection
health care economics and organizations
Pharmacology
Neurons
Memory Disorders
Brain
Choline acetyltransferase
humanities
Endocrinology
nervous system
chemistry
Psychology
Neuroscience
Acetylcholine
medicine.drug
Subjects
Details
- ISSN :
- 18790712
- Volume :
- 683
- Issue :
- 1-3
- Database :
- OpenAIRE
- Journal :
- European journal of pharmacology
- Accession number :
- edsair.doi.dedup.....91f922173456674e6a43511ef913de4a