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Analysis of Nitraria Tangutourum Bobr-Derived Fatty Acids with HPLC-FLD-Coupled Online Mass Spectrometry
- Source :
- Molecules, Volume 24, Issue 21, Molecules, Vol 24, Iss 21, p 3836 (2019)
- Publication Year :
- 2019
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2019.
-
Abstract
- Fatty acids (FAs) are basic components in plants. The pharmacological significance of FAs has attracted attentions of nutritionists and pharmaceutists. Sensitive and accurate detection of FAs is of great importance. In the present study, a pre-column derivatization and online mass spectrometry-based qualitative and quantitative analysis of FAs was developed. Nineteen main FAs were derivatized by 2-(7-methyl-1H-pyrazolo-[3,4-b]quinoline-1-yl)ethyl-4-methyl benzenesulfonate (NMP) and separated on reversed-phase Hypersil BDS C8 column with gradient elution. All FAs showed excellent linear responses with correlation coefficients more than 0.9996. The method obtained LOQs between 0.93 ng/mL and 5.64 ng/mL. FA derivatives were identified by both retention time and protonated molecular ion corresponding to m/z [M + H]+. A comparative study based on FA contents in peel and pulp, seeds and leaves of Nitraria tangutourum Bobr (NTB) from different geographical origins was performed with the established method. Results indicated that NTB were rich in FAs, and the types and contents of FAs varied among tissues. On the other hand, the same tissue of NTB from different geographical areas differed in the content, but not in type, of FAs.
- Subjects :
- nitraria tangutourum bobr
different geographical origins
Pharmaceutical Science
Protonation
Mass spectrometry
01 natural sciences
fatty acids
Article
Mass Spectrometry
Analytical Chemistry
Nitraria
lcsh:QD241-441
03 medical and health sciences
chemistry.chemical_compound
lcsh:Organic chemistry
Drug Discovery
Hplc fld
HPLC-FLD
Physical and Theoretical Chemistry
Derivatization
Nitraria tangutourum Bobr
Chromatography, High Pressure Liquid
030304 developmental biology
0303 health sciences
Chromatography
biology
010401 analytical chemistry
Organic Chemistry
biology.organism_classification
0104 chemical sciences
Plant Leaves
online mass spectrometry
chemistry
Chemistry (miscellaneous)
Seeds
Molecular Medicine
Gradient elution
Zygophyllaceae
Quantitative analysis (chemistry)
Retention time
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....91db3c8c01a5b12a4e593d03059a585b
- Full Text :
- https://doi.org/10.3390/molecules24213836