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Paramutation-like interaction of T-DNA loci in Arabidopsis
- Source :
- PLoS ONE, Vol 7, Iss 12, p e51651 (2012), PLoS ONE, PLoS ONE, Public Library of Science, 2012, 7 (12), ⟨10.1371/journal.pone.0051651⟩, PLoS One, Plos One 12 (7), . (2012)
- Publication Year :
- 2012
- Publisher :
- Public Library of Science (PLoS), 2012.
-
Abstract
- In paramutation, epigenetic information is transferred from one allele to another to create a gene expression state which is stably inherited over generations. Typically, paramutation describes a phenomenon where one allele of a gene down-regulates the expression of another allele. Paramutation has been described in several eukaryotes and is best understood in plants. Here we describe an unexpected paramutation-like trans SALK T-DNA interaction in Arabidopsis. Unlike most of the previously described paramutations, which led to gene silencing, the trans SALK T-DNA interaction caused an increase in the transcript levels of the endogenous gene (COBRA) where the T-DNA was inserted. This increased COBRA expression state was stably inherited for several generations and led to the partial suppression of the cobra phenotype. DNA methylation was implicated in this trans SALK T-DNA interaction since mutation of the DNA methyltransferase 1 in the suppressed cobra caused a reversal of the suppression. In addition, null mutants of the DNA demethylase ROS1 caused a similar COBRA transcript increase in the cobra SALK T-DNA mutant as the trans T-DNA interaction. Our results provide a new example of a paramutation-like trans T-DNA interaction in Arabidopsis, and establish a convenient hypocotyl elongation assay to study this phenomenon. The results also alert to the possibility of unexpected endogenous transcript increase when two T-DNAs are combined in the same genetic background. Citation: Xue W, Ruprecht C, Street N, Hematy K, Chang C, et al. (2012) Paramutation-Like Interaction of T-DNA Loci in Arabidopsis. PLoS ONE 7(12): e51651. doi:10.1371/journal.pone.0051651
- Subjects :
- 0106 biological sciences
EPIGENETIC INHERITANCE
[SDV]Life Sciences [q-bio]
Mutant
Arabidopsis
Plant Science
Plant Genetics
01 natural sciences
Epigenesis, Genetic
RNA-POLYMERASE
Cell Wall
Gene Expression Regulation, Plant
R1 LOCUS
ZEA-MAYS
DNA Modification Methylases
Genetics
Regulation of gene expression
0303 health sciences
Multidisciplinary
Nuclear Proteins
ORIENTED CELL EXPANSION
Phenotype
Multigene Family
DNA methylation
DEFENSE RESPONSES
Codon, Terminator
Medicine
Epigenetics
Research Article
DNA, Bacterial
Arabidopsis Thaliana
Plant Cell Biology
Science
Biology
Genes, Plant
Paramutation
03 medical and health sciences
ANCHORED PROTEIN
Model Organisms
Plant and Algal Models
Gene Silencing
RNA, Messenger
Allele
Genetik
Cellulose
Gene
Transgenic Plants
Alleles
Crosses, Genetic
030304 developmental biology
METHYLATION PATTERNS
CYTOSINE METHYLATION
MAIZE
oriented cell expansion
methylation patterns
epigenetic inheritance
cytosine methylation
defense responses
anchored protein
rna-polymerase
r1 locus
zea-mays
maize
Arabidopsis Proteins
Genetic Complementation Test
biology.organism_classification
Mutation
Plant Biotechnology
Plant Cell Wall
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 7
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....91d313d229f44b584d20dc280a658c31