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The fluorescent dyes TO-PRO-3 and TOTO-3 iodide allow detection of microbial cells in soil samples without interference from background fluorescence
- Source :
- BioTechniques. 51:190-192
- Publication Year :
- 2011
- Publisher :
- Future Science Ltd, 2011.
-
Abstract
- Visualization of microorganisms in soils and sediments using fluorescent dyes is a common method in microbial ecology studies, but is often ham- pered by strong nonspecific background fluorescence that can mask genu- ine cellular signals. The cyanine nucleic acid binding dyes TO-PRO-3 and TOTO-3 iodide enabled a clear detection of microbial cells in a mineral soil, while nonspecific background was greatly reduced compared with com- monly used dyes. When used as counterstains for fluorescence in situ hybrid- ization (FISH), both cyanine dyes allowed identification of microbial cells despite strong background from nonspecifically bound probes. TO-PRO-3 and TOTO-3 are easy to use and represent superior alternatives for detecting microorganisms in soil environments.
- Subjects :
- chemistry.chemical_classification
Background fluorescence
Chromatography
Bacteria
Microorganism
Iodide
Common method
Carbocyanines
Fluorescence
General Biochemistry, Genetics and Molecular Biology
Thiazoles
chemistry
Microbial ecology
Quinolines
Fish
In Situ Hybridization, Fluorescence
Soil Microbiology
Fluorescent Dyes
Biotechnology
Subjects
Details
- ISSN :
- 19409818 and 07366205
- Volume :
- 51
- Database :
- OpenAIRE
- Journal :
- BioTechniques
- Accession number :
- edsair.doi.dedup.....91c6b63a8e8754c8925109747ade3c2e