Back to Search
Start Over
Development of an improved selective agar medium for isolation of Yersinia pestis
- Source :
- Applied and environmental microbiology. 69(10)
- Publication Year :
- 2003
-
Abstract
- Existing media designed for selective isolation of clinically important members of the genus Yersinia were found to be unsatisfactory for the growth and isolation of Yersinia pestis . We report the development of a new selective agar medium (termed BIN) that supports the growth of Y. pestis . The development of the formulation of this medium was based on a fluorescence screening system designed for monitoring bacterial growth on semisolid media, using a green fluorescent protein-expressing strain. High-throughput combinatorial experiments can be conducted for the quantitative evaluation of the effect of different medium components on growth. Generation of fluorescence plots in this system, using microplates, allowed the quantitative evaluation of the growth rate of Y. pestis EV76 cultures in different agar compositions. The final BIN formulation is based on brain heart infusion agar, to which the selective agents irgasan, cholate salts, crystal violet, and nystatin were introduced. It was found that BIN agar is more efficient in supporting colony formation and recovery of Y. pestis than are the conventional semisolid media MacConkey agar and Yersinia -selective agar (cefsulodin-irgasan-novobiocin agar). The advantage of BIN over other media has been also demonstrated in recovering virulent Y. pestis from the mixed bacterial populations found in decaying carcasses of infected mice. The BIN medium is suggested as a selective medium for isolation and recovery of Y. pestis from various backgrounds.
- Subjects :
- food.ingredient
Yersinia pestis
education
Green Fluorescent Proteins
Colony Count, Microbial
Bacterial growth
Applied Microbiology and Biotechnology
Microbiology
Agar plate
chemistry.chemical_compound
Mice
food
Animals, Outbred Strains
Methods
Agar
Animals
Bacteriological Techniques
Plague
Ecology
biology
food and beverages
biology.organism_classification
Isolation (microbiology)
Enterobacteriaceae
Culture Media
Luminescent Proteins
chemistry
Female
MacConkey agar
Bacteria
Food Science
Biotechnology
Subjects
Details
- ISSN :
- 00992240
- Volume :
- 69
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Applied and environmental microbiology
- Accession number :
- edsair.doi.dedup.....91c2a0589763d6d94a2639759ad2c9d9