Back to Search
Start Over
Relationship Between Neutralizing Antibodies Against Adeno-Associated Virus in the Vitreous and Serum: Effects on Retinal Gene Therapy
- Source :
- Translational Vision Science & Technology
- Publication Year :
- 2019
- Publisher :
- Association for Research in Vision and Ophthalmology (ARVO), 2019.
-
Abstract
- Purpose We determine the prevalence of neutralizing antibodies (NAbs) to adeno-associated virus (AAV) in the vitreous humor and serum of patients with vitreoretinal diseases and investigate the relationship between NAb titers in the vitreous humor and serum. Methods We analyzed NAbs to AAV serotypes 2, 5, 8, and 9 via in vitro neutralization in the vitreous humor and serum from 32 patients requiring vitrectomy for vitreoretinal diseases. The blood-retinal barrier (BRB) was evaluated for integrity based on preoperative examinations, with vitreous hemorrhage (VH) on funduscopy or dye leakage on fluorescein angiography observed indicating disruption. Results NAb levels were much lower in the vitreous humor than in the serum regardless of serotype. Patients with VH had higher levels of NAbs in the vitreous humor than those without VH. The NAb ratio (ratio between NAb titers in the serum and vitreous humor) was much lower in patients with epiretinal membrane with than in those without leakage. A significantly lower NAb ratio was noticed in patients with than in those without BRB disruptions. Conclusions The NAb ratio between levels in serum and vitreous humor varies according to the condition of the BRB. Therefore, in addition to measuring the serum NAb level, physicians should examine BRB integrity when planning retinal gene therapy. Translational relevance This study provides substantial basis for retinal gene therapy using AAVs and how maintenance of BRB integrity in target diseases should be considered.
- Subjects :
- medicine.medical_specialty
genetic structures
medicine.medical_treatment
Biomedical Engineering
Blood–retinal barrier
Vitrectomy
medicine.disease_cause
chemistry.chemical_compound
Ophthalmology
medicine
Adeno-associated virus
blood–retinal barrier
medicine.diagnostic_test
biology
business.industry
neutralizing antibody
Retinal
Articles
Fluorescein angiography
medicine.disease
vitreous humor
gene therapy
eye diseases
medicine.anatomical_structure
chemistry
adeno-associated virus serotype
Vitreous hemorrhage
biology.protein
sense organs
Epiretinal membrane
Antibody
business
serum
Subjects
Details
- ISSN :
- 21642591
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Translational Vision Science & Technology
- Accession number :
- edsair.doi.dedup.....91ae7c528f580cc6a472d9c4e3930552