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Exploring the Possible Role of Lysine Acetylation on Entamoeba histolytica Virulence: A Focus on the Dynamics of the Actin Cytoskeleton
- Source :
- BioMed Research International, BioMed Research International, Vol 2013 (2013)
- Publication Year :
- 2013
- Publisher :
- Hindawi Publishing Corporation, 2013.
-
Abstract
- Cytoskeleton remodeling can be regulated, among other mechanisms, by lysine acetylation. The role of acetylation on cytoskeletal and other proteins ofEntamoeba histolyticahas been poorly studied. Dynamic rearrangements of the actin cytoskeleton are crucial for amebic motility and capping formation, processes that may be effective means of evading the host immune response. Here we report the possible effect of acetylation on the actin cytoskeleton dynamics andin vivovirulence ofE. histolytica. Using western blot, immunoprecipitation, microscopy assays, andin silicoanalysis, we show results that strongly suggest that the increase in Aspirin-induced cytoplasm proteins acetylation reduced cell movement and capping formation, likely as a consequence of alterations in the structuration of the actin cytoskeleton. Additionally, intrahepatic inoculation of Aspirin-treated trophozoites in hamsters resulted in severe impairment of the amebic virulence. Taken together, these results suggest an important role for lysine acetylation in amebic invasiveness and virulence.
- Subjects :
- Male
Cytochalasin D
Article Subject
Movement
Lysine
Molecular Sequence Data
Virulence
Arp2/3 complex
lcsh:Medicine
macromolecular substances
General Biochemistry, Genetics and Molecular Biology
Polymerization
Entamoeba histolytica
Cricetinae
Animals
Parasites
Amino Acid Sequence
Trophozoites
Cytoskeleton
Binding Sites
General Immunology and Microbiology
biology
Aspirin
lcsh:R
Acetylation
General Medicine
biology.organism_classification
Actin cytoskeleton
Actins
Cell biology
Molecular Docking Simulation
Actin Cytoskeleton
Biochemistry
Cytoplasm
biology.protein
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 23146133
- Database :
- OpenAIRE
- Journal :
- BioMed Research International
- Accession number :
- edsair.doi.dedup.....91a9e5e2cb3b02a81a6a64b716061c5c
- Full Text :
- https://doi.org/10.1155/2013/757392