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Microdiet formulation with phospholipid modulate zebrafish skeletal development and reproduction
- Source :
- Repositório Científico de Acesso Aberto de Portugal, Repositório Científico de Acesso Aberto de Portugal (RCAAP), instacron:RCAAP
- Publication Year :
- 2020
- Publisher :
- Mary Ann Liebert, 2020.
-
Abstract
- Dietary phospholipids' (PLs) content, origin, and profile are known to affect fish development and reproductive performance, but their effects in zebrafish (Danio rerio) nutrition are still poorly investigated. Therefore, this study aimed to assess the effect of practical microdiets containing plant-based and marine PL sources in zebrafish growth, survival, skeletal development, and reproductive performance. Reproductive performance was evaluated according to sperm motility, number of eggs, egg morphometry, hatching rate, and offspring standard length at 5 days postfertilization (dpf). For this purpose, seven microdiets were used, where two control diets were tested along with a supplementation with soybean lecithin (SL) as a plant-based PL source, and krill oil (KO) and copepod oil (CO) as marine PL sources, or in combinations (SLCO and SLKO). KO supplementation decreased larval growth performance and induced severe skeletal anomalies. SL supplementation reduced sperm total motility but improved offspring length at 5 dpf. CO supplementation increased sperm motility and the number of spawned eggs. Our results showed that a careful selection of the origin of dietary PL sources for microdiet formulation is critical to ensure adequate skeletal development and reproductive success. This study contributes to the improvement of zebrafish microdiet formulation and optimization of zebrafish husbandry practices. FEDER through PO Algarve 21 [23000-ZEBRAFEEDS] Portuguese Foundation for Science and Technology (FCT)Portuguese Foundation for Science and Technology [SFRH/BD/97466/2013] Foundation for Science and Technology (FCT)Portuguese Foundation for Science and Technology [UID/Multi/04326/2019] CRESC Algarve 2020 [EMBRC.PT ALG-01-0145-FEDER-022121] COMPETE 2020 [EMBRC.PT ALG-01-0145-FEDER-022121] info:eu-repo/semantics/publishedVersion
- Subjects :
- Male
Common carp
Random Allocation
0302 clinical medicine
Cyprinus-carpio
Animal Husbandry
Danio-Rerio Hamilton
Zebrafish
Sperm motility
Phospholipids
media_common
0303 health sciences
Thermal acclimation
Reproduction
Lipid composition
Fatty acidcomposition
Arachidonic acid
Fish development
embryonic structures
Sperm Motility
Animal Nutritional Physiological Phenomena
Female
animal structures
Offspring
media_common.quotation_subject
Skeletal anomalies
Biology
Krill oil
Dietary phospholipids
Andrology
03 medical and health sciences
Animals
Sperm cryopreservation
Skeleton
Ovum
030304 developmental biology
Danio rerio
Hatching
fungi
Quality control
biology.organism_classification
Animal Feed
Sperm
Diet
Animal Science and Zoology
030217 neurology & neurosurgery
Developmental Biology
Microdiets
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Repositório Científico de Acesso Aberto de Portugal, Repositório Científico de Acesso Aberto de Portugal (RCAAP), instacron:RCAAP
- Accession number :
- edsair.doi.dedup.....9195d13fb09ead901a0af759e7450e8b