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Supplementary Figures 1-7 & Supplementary Tables 1-4 from Transcriptomic Analysis of Diffuse Intrinsic Pontine Glioma (DIPG) Identifies a Targetable ALDH-Positive Subset of Highly Tumorigenic Cancer Stem-like Cells
- Publication Year :
- 2023
- Publisher :
- American Association for Cancer Research (AACR), 2023.
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Abstract
- Supplemental Figure S1. FACS analysis of Aldefluor stained SU-DIPG XIII cells. Supplemental Figure S2: Downstream signaling changes by inhibition of MAPK and PI3K/mTOR in DIPG cells. Supplemental Figure S3. Bru-sequencing transcriptome analysis identifies gene sets regulated by MAPK and PI3K/mTOR inhibition in ALDH positive SU-DIPG XIII cells. Supplemental Figure S4. A-C. Heatmaps of Bru-seq RPKM values in single agent treated (901 or GSK) ALDH+ SU-DIPG XII cells highlight treatment effects on "stemness" and DDR genes. Supplemental Figure S5: A.-B. Illustrative result of BOILED-Egg predictive model for GSK-458 and GDC-0084, respectively. Supplemental Figure S6: A-C. SU-DIPG-XIII cells were Aldefluor stained and sorted into an ALDH+ and ALDH- population. Supplemental Figure S7: A. Aldefluor stain and FACS of SU-DIPG XIII cells. B. ALDH+ cells were treated with 50 nM 901+GSK each and 24 hrs later Aldefluor re-stained and analyzed by flow cytometry. Table S1. Characteristics of Patient-Derived Diffuse Intrinsic Pontine Glioma (DIPG) Cells. Table S2. Selected Genes Upregulated in ALDH+ DIPG cells Table S3. Selected Metabolome Genes Upregulated in ALDH+ DIPG Cells. Table S4. RT-qPCR primer sequences.
Details
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....9191899f7d24ab40f99d33633b09526b