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Comparisons of Ethanol Extracts of Chinese Propolis (Poplar Type) and Poplar Gums Based on the Antioxidant Activities and Molecular Mechanism
- Source :
- Evidence-Based Complementary and Alternative Medicine, Vol 2015 (2015), Evidence-based Complementary and Alternative Medicine : eCAM
- Publication Year :
- 2015
- Publisher :
- Hindawi Limited, 2015.
-
Abstract
- The biological activities of propolis are varied from plant sources and the prominent antioxidant effects of Chinese propolis (poplar type) have been extensively reported. Oxidative stress is associated with inflammation and induces many diseases. In the study, to evaluate antioxidant capacities and clarify the underlying molecular mechanisms of ethanol extracts of Chinese propolis (EECP) and ethanol extracts of poplar gums (EEPG), we analyzed their compositions by HPLC, evaluating their free radical scavenging activities and reducing power by chemical analysis methods. Moreover, we studied the roles of EECP and EEPG on the elimination of ROS and expressions of antioxidant genes (HO-1, TrxR1, GCLM, and GCLC) in RAW264.7 cells. We further investigated the effects of MAPKs on the antioxidant genes expression by specific inhibitors. The nucleus translocation effects of Nrf2 were also measured by confocal microscopy analysis. The results indicated that EECP had higher TPC and FDC values but regarding TFC values were not significant. EECP also possessed more contents of 11 compounds than EEPG. Both phytochemical analysis and cell experiment reflected that EECP exerted stronger antioxidant activities than EEPG. EECP and EEPG enhanced endogenous antioxidant defenses by eliminating reactive oxygen species directly and activating Erk-Nrf2-HO1, GCLM, and TrxR1 signal pathways.
- Subjects :
- chemistry.chemical_classification
Reactive oxygen species
Antioxidant
Article Subject
Chemistry
GCLM
medicine.medical_treatment
Endogeny
lcsh:Other systems of medicine
Propolis
medicine.disease_cause
lcsh:RZ201-999
GCLC
Complementary and alternative medicine
Phytochemical
Botany
medicine
Food science
Oxidative stress
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 17414288
- Volume :
- 2015
- Database :
- OpenAIRE
- Journal :
- Evidence-Based Complementary and Alternative Medicine
- Accession number :
- edsair.doi.dedup.....918b516e684d8f798548d00b25354031