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The ontogeny of complement component C3 in the spotted wolffish (Anarhichas minor Olafsen)
- Source :
- Fish & Shellfish Immunology. 18:351-358
- Publication Year :
- 2005
- Publisher :
- Elsevier BV, 2005.
-
Abstract
- Eggs and larvae from the spotted wolffish (Anarhichas minor Olafsen) were analysed to study the ontogeny of the complement component C3. By Western blotting experiments, the results revealed that C3 was present already in unfertilised eggs and that there was a decrease in C3 content towards hatch, whereas the results using reverse transcription-polymerase chain-reaction showed a continuous increase of C3 mRNA content towards hatch. These findings indicate a transfer of maternal complement component C3 to the offspring where the content of C3 decreases and the level of C3 mRNA increases towards hatch. Immunohistochemical experiments revealed that only the liver of spotted wolffish larvae contained complement component C3, suggesting that the liver is the main site of production.
- Subjects :
- animal structures
Offspring
Ontogeny
Blotting, Western
Zoology
Aquatic Science
Biology
Anarhichas
Animals
Environmental Chemistry
RNA, Messenger
Ovum
Larva
Reverse Transcriptase Polymerase Chain Reaction
Ecology
Spotted wolffish
Temperature
Complement C3
General Medicine
biology.organism_classification
Immunohistochemistry
Perciformes
Liver metabolism
Liver
Fish
Subjects
Details
- ISSN :
- 10504648
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- Fish & Shellfish Immunology
- Accession number :
- edsair.doi.dedup.....918055980cb1041c3438e43645c4eb13
- Full Text :
- https://doi.org/10.1016/j.fsi.2004.09.002