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Differentiation of the granulosa layer from hen prehierarchal follicles associated with follicle-stimulating hormone receptor signaling
- Source :
- Molecular reproduction and development. 85(8-9)
- Publication Year :
- 2018
-
Abstract
- Recruitment of a single follicle into the preovulatory hierarchy of the domestic hen ovary occurs from a small cohort of prehierarchal follicles measuring 6-8 mm in diameter. We have previously reported that granulosa cells (GCs) collected from prehierarchal follicles express highest levels of membrane-localized follicle-stimulating hormone receptor (FSHR) during follicle development, yet fail to initiate signaling via cAMP following short-term incubation with FSH. Consequently, GC from prehierarchal follicles remain in an undifferentiated state and lack the capacity for steroidogenesis due to a deficiency of cAMP-dependent STAR protein and CYP11A1 gene expression. The present studies investigate FSH responsiveness in GC before and after the transition from undifferentiated to a differentiated state at follicle recruitment. Before recruitment focus is directed toward the inhibition of FSHR-signaling by β-ARRESTIN (βARR). Specifically, knockdown of βARR messenger RNA in cultured, undifferentiated GC using small interfering RNA facilitated FSH-induced cAMP formation, STAR expression, and progesterone production. Furthermore, overexpression of bovine βARR1 and G-protein-coupled receptor kinase2 in actively differentiating GC significantly decreased cAMP accumulation and progesterone production following a challenge with FSH. We propose that a βARR-mediated mechanism maintains FSHR unresponsiveness in undifferentiated GC from prehierarchal follicles, and as a result prevents GC differentiation until the time of follicle recruitment.
- Subjects :
- 0301 basic medicine
endocrine system
Ovary
Biology
Transfection
03 medical and health sciences
Follicle
Follicle-stimulating hormone
Ovarian Follicle
Gene expression
Genetics
medicine
Animals
Cholesterol Side-Chain Cleavage Enzyme
RNA, Messenger
Receptor
Cells, Cultured
Progesterone
Messenger RNA
Analysis of Variance
Granulosa Cells
Membrane Transport Proteins
Cell Differentiation
Cell Biology
Cell biology
030104 developmental biology
medicine.anatomical_structure
Hormone receptor
Gene Knockdown Techniques
Receptors, FSH
Cattle
Female
Follicle Stimulating Hormone
Mitogen-Activated Protein Kinases
Follicle-stimulating hormone receptor
Chickens
Developmental Biology
Signal Transduction
Subjects
Details
- ISSN :
- 10982795
- Volume :
- 85
- Issue :
- 8-9
- Database :
- OpenAIRE
- Journal :
- Molecular reproduction and development
- Accession number :
- edsair.doi.dedup.....916c3fefb0188ac8ac8a7837de9ba90f