Back to Search
Start Over
Single-cell profiling of the human decidual immune microenvironment in patients with recurrent pregnancy loss
Single-cell profiling of the human decidual immune microenvironment in patients with recurrent pregnancy loss
- Source :
- Cell Discovery, Vol 7, Iss 1, Pp 1-15 (2021), Cell Discovery
- Publication Year :
- 2021
- Publisher :
- Nature Publishing Group, 2021.
-
Abstract
- Maintaining homeostasis of the decidual immune microenvironment at the maternal–fetal interface is essential for placentation and reproductive success. Although distinct decidual immune cell subpopulations have been identified under normal conditions, systematic understanding of the spectrum and heterogeneity of leukocytes under recurrent miscarriage in human deciduas remains unclear. To address this, we profiled the respective transcriptomes of 18,646 primary human decidual immune cells isolated from patients with recurrent pregnancy loss (RPL) and healthy controls at single-cell resolution. We discovered dramatic differential distributions of immune cell subsets in RPL patients compared with the normal decidual immune microenvironment. Furthermore, we found a subset of decidual natural killer (NK) cells that support embryo growth were diminished in proportion due to abnormal NK cell development in RPL patients. We also elucidated the altered cellular interactions between the decidual immune cell subsets in the microenvironment and those of the immune cells with stromal cells and extravillous trophoblast under disease state. These results provided deeper insights into the RPL decidual immune microenvironment disorder that are potentially applicable to improve the diagnosis and therapeutics of this disease.
- Subjects :
- Stromal cell
Cell
Immunology
chemical and pharmacologic phenomena
Biology
Biochemistry
Article
Transcriptome
03 medical and health sciences
0302 clinical medicine
Immune system
Recurrent miscarriage
Genetics
medicine
lcsh:QH573-671
Molecular Biology
030304 developmental biology
0303 health sciences
Cell growth
lcsh:Cytology
Placentation
Embryo
Cell Biology
biochemical phenomena, metabolism, and nutrition
medicine.disease
Gene expression profiling
medicine.anatomical_structure
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 20565968
- Volume :
- 7
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Cell Discovery
- Accession number :
- edsair.doi.dedup.....916aa5c05d5d10a637aef3f5ee9f1763