Back to Search
Start Over
Identification and expansion of human osteosarcoma-cancer-stem cells by long-term 3-aminobenzamide treatment
- Source :
- Journal of Cellular Physiology. 219:301-313
- Publication Year :
- 2009
- Publisher :
- Wiley, 2009.
-
Abstract
- A novel cancer stem-like cell line (3AB-OS), expressing a number of pluripotent stem cell markers, was irreversibly selected from human osteosarcoma MG-63 cells by long-term treatment (100 days) with 3-aminobenzamide (3AB). 3AB-OS cells are a heterogeneous and stable cell population composed by three types of fibroblastoid cells, spindle-shaped, polygonal-shaped, and rounded-shaped. With respect to MG-63 cells, 3AB-OS cells are extremely smaller, possess a much greater capacity to form spheres, a stronger self-renewal ability and much higher levels of cell cycle markers which account for G1-S/G2-M phases progression. Differently from MG-63 cells, 3AB-OS cells can be reseeded unlimitedly without losing their proliferative potential. They show an ATP-binding cassette transporter ABCG2-dependent phenotype with high drug efflux capacity, and a strong positivity for CD133, marker for pluripotent stem cells, which are almost unmeasurable in MG-63 cells. 3AB-OS cells are much less committed to osteogenic and adipogenic differentiation than MG-63 cells and highly express genes required for maintaining stem cell state (Oct3/4, hTERT, nucleostemin, Nanog) and for inhibiting apoptosis (HIF-1alpha, FLIP-L, Bcl-2, XIAP, IAPs, and survivin). 3AB-OS may be a novel tumor cell line useful for investigating the mechanisms by which stem cells enrichment may be induced in a tumor cell line. The identification of a subpopulation of cancer stem cells that drives tumorigenesis and chemoresistance in osteosarcoma may lead to prognosis and optimal therapy determination. Expression patterns of stem cell markers, especially CD133 and ABCG2, may indicate the undifferentiated state of osteosarcoma tumors, and may correlate with unfavorable prognosis in the clinical setting.
- Subjects :
- Adult
Homeobox protein NANOG
Adolescent
Physiology
Cellular differentiation
Clinical Biochemistry
Apoptosis
Biology
Stem cell marker
Young Adult
cancer stemm cells, osteosarcoma, PARP inhibitors
Cancer stem cell
Cell Line, Tumor
Settore BIO/10 - Biochimica
Humans
Rhodamine 123
Enzyme Inhibitors
Progenitor cell
Child
Induced pluripotent stem cell
Cell Shape
Cell potency
Fluorescent Dyes
Osteosarcoma
Cell Differentiation
Cell Biology
Calcium Channel Blockers
Drug Resistance, Multiple
Gene Expression Regulation, Neoplastic
Verapamil
Benzamides
Immunology
Neoplastic Stem Cells
Cancer research
ATP-Binding Cassette Transporters
Benzimidazoles
Stem cell
Biomarkers
Subjects
Details
- ISSN :
- 10974652 and 00219541
- Volume :
- 219
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular Physiology
- Accession number :
- edsair.doi.dedup.....9156ec154fdc21ebe3ad7309bb2f1327