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Barrier function of porcine choroid plexus epithelial cells is modulated by cAMP-dependent pathways in vitro
- Source :
- Brain Research. 853:115-124
- Publication Year :
- 2000
- Publisher :
- Elsevier BV, 2000.
-
Abstract
- In the present paper, we demonstrate that the barrier properties of primary cultured epithelial cells isolated from porcine choroid plexus are regulated by cAMP-dependent signal transduction pathways in vitro. Triggering cAMP-connected cascades in cell layers grown on permeable filters with cAMP-analogues or forskolin led to a significant increase of transepithelial electrical resistances and a pronounced reduction in the permeation rate of a 4 kDa-dextran probe. In dose-response experiments using the cAMP-analogue 8-(4-chlorophenylthio)-cAMP transepithelial electrical resistances were observed to increase above a threshold concentration ranging between 10(-5.5) and 10(-5) M. Additional impedance studies performed with confluent cell layers grown on gold-film electrodes revealed that the observed changes in transepithelial resistances and presumably also in macromolecular permeation rates were not entirely caused by a reinforcement of intercellular junctions but also contained contributions from changes in the cell-substrate adhesion pattern. These inherent contributions to the electrical resistance and macromolecular permeability are caused by a restricted diffusion pathway between basal plasma membrane and culture substrate that have to be considered in data analysis, especially when leaky cell layers on filter substrates with low pore densities are used.
- Subjects :
- medicine.medical_specialty
Cell Membrane Permeability
Swine
Biology
Models, Biological
Cell junction
chemistry.chemical_compound
Internal medicine
Cyclic AMP
Electric Impedance
medicine
Choroid Plexus Epithelium
Animals
Electrodes
Molecular Biology
Cells, Cultured
Barrier function
Forskolin
Dose-Response Relationship, Drug
General Neuroscience
Colforsin
Epithelial Cells
Adhesion
Thionucleotides
Permeation
Basal plasma membrane
Endocrinology
chemistry
Blood-Brain Barrier
Choroid Plexus
Biophysics
Choroid plexus
Gold
Neurology (clinical)
Signal Transduction
Developmental Biology
Subjects
Details
- ISSN :
- 00068993
- Volume :
- 853
- Database :
- OpenAIRE
- Journal :
- Brain Research
- Accession number :
- edsair.doi.dedup.....914a877e8ea002659e2b0f55e0a28b14