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The pharmacological effect of Bothrops neuwiedii pauloensis (jararaca-pintada) snake venom on avian neuromuscular transmission
- Source :
- Brazilian Journal of Medical and Biological Research v.36 n.5 2003, Brazilian Journal of Medical and Biological Research, Associação Brasileira de Divulgação Científica (ABDC), instacron:ABDC, Brazilian Journal of Medical and Biological Research, Vol 36, Iss 5, Pp 617-624 (2003), Brazilian Journal of Medical and Biological Research, Volume: 36, Issue: 5, Pages: 617-624, Published: MAY 2003
- Publication Year :
- 2003
- Publisher :
- Associação Brasileira de Divulgação Científica, 2003.
-
Abstract
- The neuromuscular effects of Bothrops neuwiedii pauloensis (jararaca-pintada) venom were studied on isolated chick biventer cervicis nerve-muscle preparations. Venom concentrations of 5-50 micro g/ml produced an initial inhibition and a secondary increase of indirectly evoked twitches followed by a progressive concentration-dependent and irreversible neuromuscular blockade. At venom concentrations of 1-20 micro g/ml, the responses to 13.4 mM KCl were inhibited whereas those to 110 micro M acetylcholine alone and cumulative concentrations of 1 micro M to 10 mM were unaffected. At venom concentrations higher than 50 micro g/ml, there was pronounced muscle contracture with inhibition of the responses to acetylcholine, KCl and direct stimulation. At 20-24 degrees C, the venom (50 g/ml) produced only partial neuromuscular blockade (30.7 +/- 8.0%, N = 3) after 120 min and the initial inhibition and the secondary increase of the twitch responses caused by the venom were prolonged and pronounced and the response to KCl was unchanged. These results indicate that B.n. pauloensis venom is neurotoxic, acting primarily at presynaptic sites, and that enzyme activity may be involved in this pharmacological action.
- Subjects :
- Time Factors
Physiology
Immunology
Neuromuscular Junction
Biophysics
Neuromuscular transmission
Chick biventer cervicis
Venom
Pharmacology
Biochemistry
Potassium Chloride
Presynaptic action
Phospholipase A2
Crotalid Venoms
medicine
Neurotoxicity
Animals
Bothrops
General Pharmacology, Toxicology and Pharmaceutics
Muscle, Skeletal
lcsh:QH301-705.5
Jararaca pintada
lcsh:R5-920
Neuromuscular Blockade
Dose-Response Relationship, Drug
biology
General Neuroscience
Cell Biology
General Medicine
Myotoxicity
biology.organism_classification
Acetylcholine
Enzyme assay
lcsh:Biology (General)
Snake venom
Anesthesia
biology.protein
lcsh:Medicine (General)
Chickens
Muscle Contraction
medicine.drug
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Brazilian Journal of Medical and Biological Research v.36 n.5 2003, Brazilian Journal of Medical and Biological Research, Associação Brasileira de Divulgação Científica (ABDC), instacron:ABDC, Brazilian Journal of Medical and Biological Research, Vol 36, Iss 5, Pp 617-624 (2003), Brazilian Journal of Medical and Biological Research, Volume: 36, Issue: 5, Pages: 617-624, Published: MAY 2003
- Accession number :
- edsair.doi.dedup.....9117b189556712e0321135a0d4404d6c