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Therapeutic effects of monoclonal antibody g250, interferons and tumor necrosis factor, in mice with renal-cell carcinoma xenografts
- Source :
- International Journal of Cancer. 56:262-268
- Publication Year :
- 1994
- Publisher :
- Wiley, 1994.
-
Abstract
- Because renal-cell carcinoma (RCC) is considered relatively resistant to radio- and chemotherapy, RCC patients may benefit from new treatment modalities, e.g. immunotherapy. In vitro and in vivo studies suggest that combinations of cytokines such as interferon gamma or interferon alpha (IFN-gamma, IFN-alpha) and tumor necrosis factor alpha (TNF-alpha) may act synergistically. In this study we tested whether a monoclonal antibody (MAb) G250, reactive with a RCC-associated antigen, showed anti-tumor effects in vivo in nude mice with established s.c. human RCC xenografts, and also whether this MAb could enhance the anti-tumor effect of combinations of IFNs and TNF-alpha. Treatment with combinations of IFN-alpha/TNF-alpha or IFN-gamma/TNF-alpha, or with MAb G250 alone, resulted in a significant inhibition of tumor growth. Treatment with MAb G250, in combination with IFN-gamma/TNF-alpha, did not result in an improve anti-tumor effect as compared to that of either treatment alone. In contrast, MAb G250 combined with IFN-alpha/TNF-alpha resulted in a significantly enhanced anti-tumor response. In one experiment, 3 out of 10 mice showed complete tumor regression, with no recurrence after 90 days. Large numbers of infiltrating macrophages were found surrounding viable and necrotic tumor tissue after treatment with G250 combined with IFN-alpha/TNF-alpha. These results suggest that combination therapy, consisting of IFN-alpha, TNF-alpha and MAbs, may have therapeutic value in the treatment of RCC.
- Subjects :
- Cancer Research
medicine.drug_class
medicine.medical_treatment
Transplantation, Heterologous
Alpha interferon
Monoclonal antibody
Interferon-gamma
Mice
Monoclonal antibody G250
Antigen
In vivo
Antineoplastic Combined Chemotherapy Protocols
medicine
Animals
Interferon gamma
Carcinoma, Renal Cell
Mice, Inbred BALB C
Tumor Necrosis Factor-alpha
business.industry
Antibodies, Monoclonal
Immunotherapy
Immunohistochemistry
Kidney Neoplasms
Recombinant Proteins
Oncology
Interferon Type I
Immunology
Cancer research
Tumor necrosis factor alpha
business
Neoplasm Transplantation
medicine.drug
Subjects
Details
- ISSN :
- 10970215 and 00207136
- Volume :
- 56
- Database :
- OpenAIRE
- Journal :
- International Journal of Cancer
- Accession number :
- edsair.doi.dedup.....9115c76d102bca6c74e656b76348eabf