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Effect of increased uptake of plasma fatty acids by the liver on lipid metabolism in the hepatocellular nuclei
- Source :
- Prostaglandins, Leukotrienes and Essential Fatty Acids. 57:27-31
- Publication Year :
- 1997
- Publisher :
- Elsevier BV, 1997.
-
Abstract
- The nucleus contains different lipids. The aim of the present study was to examine whether increased uptake of free fatty acids by the liver affects lipid metabolism in the hepatocellular nuclei. The experiments were carried out on three groups of Wistar rats: I — male, control; II — male, heparin-treated, and III — female. [14C]-palmitic acid suspended in rat donor serum was administered intravenously 5 and 30 min before tissue samples were taken. Lipids were extracted from isolated liver nuclei and separated into different fractions (phospholipids — PH, monoacylglycerols — MG, diacylglycerols — DG, cholesterol — CH, free fatty acids — FFA, triacylglycerols — TG and cholesterol esters — CHE). It was found that 5 min after administration of the label all isolated nuclear lipid fractions were radioactive. Most of the radioactivity was located in the fraction of PH, TG and FFA. Elevation in the plasma FFA concentration (heparin-treated group) resulted in increased incorporation of [14C]-palmitic acid into the nuclear lipids and changes in its distribution. In the female rats the radioactivity of nuclear lipids was higher than in the male-controls. There were also differences in the percentage distribution of the radioactivity in different lipid fractions between the two groups. The concentration of PH and TG in the nuclei increased only in the heparin-treated but not in the female rats. However, specific activity of the nuclear PH and TG increased in with both groups compared to the male-control group. It is concluded that (a) the blood-borne FFA rapidly enter the nuclear lipid pool and (b) increased uptake of the plasma-borne FFA by the liver affects the nuclear lipid metabolism.
- Subjects :
- Male
medicine.medical_specialty
Clinical Biochemistry
Palmitic Acid
Fatty Acids, Nonesterified
Biology
chemistry.chemical_compound
Internal medicine
medicine
Animals
Distribution (pharmacology)
Carbon Radioisotopes
Rats, Wistar
Phospholipids
Triglycerides
Cell Nucleus
Heparin
Cholesterol
Fatty Acids
Lipid metabolism
Cell Biology
Metabolism
Lipid Metabolism
Rats
Cell nucleus
medicine.anatomical_structure
Endocrinology
Liver
chemistry
Biochemistry
Hepatocyte
Female
lipids (amino acids, peptides, and proteins)
Specific activity
Nucleus
Subjects
Details
- ISSN :
- 09523278
- Volume :
- 57
- Database :
- OpenAIRE
- Journal :
- Prostaglandins, Leukotrienes and Essential Fatty Acids
- Accession number :
- edsair.doi.dedup.....9104f890074db7582ba39f64b6dd3017