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Dietary Emulsifiers Directly Impact Adherent-Invasive E. coli Gene Expression to Drive Chronic Intestinal Inflammation
- Source :
- Cell Reports, Vol 33, Iss 1, Pp 108229-(2020), Cell Reports, Cell Reports, 2020, 33 (1), pp.108229. ⟨10.1016/j.celrep.2020.108229⟩, Cell Reports, Elsevier Inc, 2020, 33 (1), pp.108229. ⟨10.1016/j.celrep.2020.108229⟩
- Publication Year :
- 2020
- Publisher :
- Elsevier, 2020.
-
Abstract
- Summary Dietary emulsifiers carboxymethylcellulose (CMC) and polysorbate-80 (P80) disturb gut microbiota, promoting chronic inflammation. Mice with minimal microbiota are protected against emulsifiers’ effects, leading us to hypothesize that these compounds might provoke select pathobionts to promote inflammation. Gnotobiotic wild-type (WT) and interleukin-10 (IL-10)−/− mice were colonized with Crohn’s-disease-associated adherent-invasive E. coli (AIEC) and subsequently administered CMC or P80. AIEC colonization of GF and altered Schaedler flora (ASF) mice results in chronic intestinal inflammation and metabolism dysregulations when consuming the emulsifier. In IL-10−/− mice, AIEC mono-colonization results in severe intestinal inflammation in response to emulsifiers. Exposure of AIEC to emulsifiers in vitro increases its motility and ability to adhere to intestinal epithelial cells. Transcriptomic analysis reveals that emulsifiers directly induce expression of clusters of genes that mediate AIEC virulence and promotion of inflammation. To conclude, emulsifiers promote virulence and encroachment of pathobionts, providing a means by which these compounds may drive inflammation in hosts carrying such bacteria.<br />Graphical Abstract<br />Highlights • Dietary emulsifiers alter the intestinal microbiota, promoting chronic inflammation • Select pathobionts are required to mediate the detrimental effects of emulsifiers • Emulsifiers directly induce the expression of bacterial virulence genes • Microbiota-based dietary intervention appears warranted<br />Through gnotobiotic and molecular approaches, Viennois et al. demonstrate that in mice, the ability of a dietary emulsifier to promote chronic intestinal inflammation and colitis-associated cancer is mediated by the ability of these compounds to directly induce the expression of clusters of genes that mediate virulence of a select pathobiont.
- Subjects :
- 0301 basic medicine
Motility
Virulence
Gene Expression
Inflammation
Gut flora
flagellin
General Biochemistry, Genetics and Molecular Biology
Article
Microbiology
Transcriptome
03 medical and health sciences
Mice
0302 clinical medicine
intestinal inflammation
medicine
Escherichia coli
microbiota
Animals
Humans
emulsifier
lcsh:QH301-705.5
biology
Chemistry
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology
biology.organism_classification
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
3. Good health
Altered Schaedler flora
Intestines
030104 developmental biology
adherent-invasive Escherichia coli
lcsh:Biology (General)
Emulsifying Agents
Chronic Disease
pathobiont
biology.protein
medicine.symptom
diet
030217 neurology & neurosurgery
Bacteria
Flagellin
Subjects
Details
- Language :
- English
- ISSN :
- 22111247
- Volume :
- 33
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Cell Reports
- Accession number :
- edsair.doi.dedup.....90fc74e4072b6850f3bb8ced611263cc