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Evaluation of the MicroSEQ™ Salmonella spp. Detection Kit with the PrepSEQ™ Rapid Spin Sample Preparation Kit for Detection of Salmonella spp. in Dry Pet Food
- Source :
- Journal of AOAC International. 99(2)
- Publication Year :
- 2016
-
Abstract
- A method modification validation study was conducted to validate the Applied Biosystems MicroSEQ™ Salmonella spp. Detection Kit for the detection of Salmonella spp. in 375 g samples of dried pet food. The MicroSEQ assay protocol, using the Applied Biosystems PrepSEQ™ Rapid Spin DNA Sample Preparation Kit, was compared to the reference method detailed in the U.S Food and Drug Administration (FDA) Bacteriological Analytical Manual (BAM; Chapter 5, Salmonella) for detection of Salmonella spp. For each method, 20 replicates were analyzed at a low contamination level of 0.2–2 CFU/test portion, five replicates were analyzed at a high level of contamination of 2–5 CFU/test portion, and five control replicates were also analyzed at 0 CFU/test portion (uninoculated). Statistical analysis was conducted using the Probability of Detection statistical test to determine the ability of the MicroSEQ Salmonella spp. Detection Kit to detect Salmonella from 375 g samples of dried pet food in comparison to the FDA-BAM reference method. The results demonstrated that the MicroSEQ Salmonella spp. Detection Kit was able to accurately detect Salmonella spp. present in dry pet food after an enrichment time of 20 h.
- Subjects :
- DNA, Bacterial
Salmonella
Validation study
Biology
medicine.disease_cause
Polymerase Chain Reaction
Analytical Chemistry
Microbiology
Food and drug administration
Pet food
0404 agricultural biotechnology
medicine
Environmental Chemistry
Food microbiology
Animals
Sample preparation
Food science
Pharmacology
04 agricultural and veterinary sciences
Pets
Contamination
040401 food science
Animal Feed
Food Analysis
United States
Food Microbiology
Reagent Kits, Diagnostic
Agronomy and Crop Science
Food Science
Subjects
Details
- ISSN :
- 19447922
- Volume :
- 99
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of AOAC International
- Accession number :
- edsair.doi.dedup.....90e2f8483bc83d9524805e92b7ade590