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Long-Term Implantation of Preadipocyte-Seeded PLGA Scaffolds
- Source :
- Tissue Engineering. 8:283-293
- Publication Year :
- 2002
- Publisher :
- Mary Ann Liebert Inc, 2002.
-
Abstract
- Studies were performed in a long-term effort to develop clinically translatable, tissue engineered adipose constructs for reconstructive, correctional, and cosmetic indications. Rat preadipocytes were harvested, isolated, expanded ex vivo, and seeded within PLGA scaffolds. Preadipocyte-seeded and acellular (control) scaffolds were implanted for 1-12 months. Explanted scaffolds were stained with osmium tetroxide, processed, and counterstained using HE. Quantitative histomorphometric analysis was performed on all tissue sections to determine the amount of adipose tissue formed. Analyses revealed maximum adipose formation at 2 months, followed by a decrease at 3 months, and complete absence of adipose and PLGA at 5-12 months. These results extend a previous short-term study (Tissue Engineering 1999;5:134) and demonstrate that adipose tissue can be formed in vivo using tissue engineering strategies. However, the long-term maintenance of adipose tissue remains elusive.
- Subjects :
- Male
Time Factors
Tissue engineered
Tissue Engineering
Polymers
Chemistry
General Engineering
Adipose tissue
Biocompatible Materials
Rats
chemistry.chemical_compound
PLGA
Adipose Tissue
Polylactic Acid-Polyglycolic Acid Copolymer
Osmium tetroxide
Tissue engineering
Rats, Inbred Lew
In vivo
Adipocytes
Animals
Lactic Acid
Polyglycolic Acid
Ex vivo
Adipose tissue engineering
Biomedical engineering
Subjects
Details
- ISSN :
- 15578690 and 10763279
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Tissue Engineering
- Accession number :
- edsair.doi.dedup.....90d44fc51254744acbcea20afdac9d50