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Occurrence of monosialosyl pentahexaosylceramide GalNAc-GM1 as specific tumor-associated ganglioside of human head and neck squamous cell carcinomas
- Source :
- Cancer Letters. 135:159-164
- Publication Year :
- 1999
- Publisher :
- Elsevier BV, 1999.
-
Abstract
- In a recent study of the ganglioside profiles of human head and neck squamous cell carcinomas versus normal tissue, one unidentified GX ganglioside was found exclusively in tumor extracts, migrating between GM1 and GD3 by thin-layer chromatography. To determine the chemical structure of this ganglioside which accounted for 3-8% of the total gangliosides, the lipid samples were pooled and separated by high-pressure liquid chromatography to obtain individual ganglioside species purified to homogeneity. The tumor-associated GX ganglioside was analyzed by gas-liquid chromatography, mass spectrometry and immunostaining on thin-layer plates with mouse monoclonal antibodies after enzymatic cleavage. The data allowed the identification of GX ganglioside as GalNAc-GM1 that has been reported as a very minor brain ganglioside in humans. Thus, GalNAc-GM1 is a specific tumor-associated ganglioside in human head and neck squamous cell carcinomas that could be potentially valuable for clinicians.
- Subjects :
- Cancer Research
Chromatography, Gas
medicine.drug_class
Cell
Normal tissue
G(M1) Ganglioside
Monoclonal antibody
Mass Spectrometry
medicine
Humans
chemistry.chemical_classification
Ganglioside
Chemistry
Molecular biology
In vitro
Neoplasm Proteins
Enzyme
medicine.anatomical_structure
Oncology
Epidermoid carcinoma
Biochemistry
Head and Neck Neoplasms
Carcinoma, Squamous Cell
N-Acetylgalactosaminyltransferases
lipids (amino acids, peptides, and proteins)
Chromatography, Thin Layer
Immunostaining
Subjects
Details
- ISSN :
- 03043835
- Volume :
- 135
- Database :
- OpenAIRE
- Journal :
- Cancer Letters
- Accession number :
- edsair.doi.dedup.....90c2cf9b09c4ffc45ee8ff8e9b702a03