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Cryoelectron Microscopy Maps of Human Papillomavirus 16 Reveal L2 Densities and Heparin Binding Site
- Source :
- Structure. 25:253-263
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- Human papillomavirus (HPV) is a significant health burden and leading cause of virus-induced cancers. The current commercial vaccines are genotype specific and provide little therapeutic benefit to patients with existing HPV infections. Host entry mechanisms represent an excellent target for alternative therapeutics, but HPV receptor use, the details of cell attachment, and host entry are inadequately understood. Here we present near-atomic resolution structures of the HPV16 capsid and HPV16 in complex with heparin, both determined from cryoelectron micrographs collected with direct electron detection technology. The structures clarify details of capsid architecture for the first time, including variation in L1 major capsid protein conformation and putative location of L2 minor protein. Heparin binds specifically around the capsid icosahedral vertices and may recapitulate the earliest stage of infection, providing a framework for continuing biochemical, genetic, and biophysical studies.
- Subjects :
- Models, Molecular
Protein Conformation, alpha-Helical
0301 basic medicine
Cryo-electron microscopy
viruses
Amino Acid Motifs
030106 microbiology
Gene Expression
Plasma protein binding
Biology
Crystallography, X-Ray
03 medical and health sciences
Capsid
Protein structure
Virus-like particle
Structural Biology
Genotype
Humans
Protein Interaction Domains and Motifs
Cloning, Molecular
Binding site
Molecular Biology
Human papillomavirus 16
Binding Sites
Heparin
Cryoelectron Microscopy
HEK 293 cells
Oncogene Proteins, Viral
Virology
Recombinant Proteins
HEK293 Cells
030104 developmental biology
Capsid Proteins
Protein Conformation, beta-Strand
Protein Binding
Subjects
Details
- ISSN :
- 09692126
- Volume :
- 25
- Database :
- OpenAIRE
- Journal :
- Structure
- Accession number :
- edsair.doi.dedup.....90c1e24103a31ee208a97c4dd9b3385e