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Proteomic and Metabolomic Characterization of COVID-19 Patient Sera
- Source :
- Cell
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- Summary Early detection and effective treatment of severe COVID-19 patients remain major challenges. Here, we performed proteomic and metabolomic profiling of sera from 46 COVID-19 and 53 control individuals. We then trained a machine learning model using proteomic and metabolomic measurements from a training cohort of 18 non-severe and 13 severe patients. The model was validated using 10 independent patients, 7 of which were correctly classified. Targeted proteomics and metabolomics assays were employed to further validate this molecular classifier in a second test cohort of 19 COVID-19 patients, leading to 16 correct assignments. We identified molecular changes in the sera of COVID-19 patients compared to other groups implicating dysregulation of macrophage, platelet degranulation, complement system pathways, and massive metabolic suppression. This study revealed characteristic protein and metabolite changes in the sera of severe COVID-19 patients, which might be used in selection of potential blood biomarkers for severity evaluation.<br />Graphical Abstract<br />Highlights • 93 proteins show differential expression in severe COVID-19 patient sera • 204 metabolites in COVID-19 patient sera correlate with disease severity • A model composed of 29 serum factors shows patient stratification potential • Pathway analysis highlights metabolic and immune dysregulation in COVID-19 patients<br />Proteomic and metabolomic analysis of COVID-19 sera identifies differentially expressed factors that correlate with disease severity and highlights dysregulation of multiple immune and metabolic components in clinically severe patients.
- Subjects :
- Oncology
Male
Proteomics
Metabolite
Serum protein
severity
Disease
Severity of Illness Index
Machine Learning
chemistry.chemical_compound
0302 clinical medicine
Platelet degranulation
Medicine
Cluster Analysis
Amino Acids
0303 health sciences
Middle Aged
metabolomics
Pathophysiology
Cohort
Female
Coronavirus Infections
Adult
medicine.medical_specialty
Coronavirus disease 2019 (COVID-19)
Pneumonia, Viral
Early detection
macromolecular substances
Biology
Article
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
Metabolomics
Internal medicine
Severity of illness
Effective treatment
Humans
Pandemics
030304 developmental biology
business.industry
Macrophages
COVID-19
Lipid Metabolism
Training cohort
Complement system
chemistry
Immunology
business
serum
Biomarkers
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 00928674
- Database :
- OpenAIRE
- Journal :
- Cell
- Accession number :
- edsair.doi.dedup.....90be2283a21bfbfecc8f9f1396ba0900
- Full Text :
- https://doi.org/10.1016/j.cell.2020.05.032