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Activation of cell cycle regulatory proteins in the apoptosis of terminally differentiated oligodendrocytes
- Source :
- Neurochemical research 29 (2004): 923–931., info:cnr-pdr/source/autori:Strazza M., Luddi, A.., Brogi A., Ricci M., Santi S., Melli M., Costantino-Ceccarini E./titolo:Activation of cell cycle regulatory proteins in the apoptosis of terminally differentiated oligodendrocytes/doi:/rivista:Neurochemical research/anno:2004/pagina_da:923/pagina_a:931/intervallo_pagine:923–931/volume:29
- Publication Year :
- 2004
-
Abstract
- There is increasing evidence that proteins normally involved in the cell cycle play a role in the regulation of neuronal apoptotic death following various insults. However, it is not clear if the same mechanisms regulate cell death of oligodendrocytes as well. In this study, we investigated the mechanism of ceramide-induced apoptosis in primary rat oligodendrocytes. We show that ceramide treatment initiates a cascade of biochemical events involving cell cycle regulatory proteins. Although at the time of induction of cell death the oligodendrocytes are postmitotic, activation of c-myc and translocation of Cdc25A into the nucleus can be demonstrated. Of particular interest are the findings of the up-regulation of PCNA and down-regulation of p21WAF1/CIP1 protein, an inhibitor of cell-cycle progression. The current results show that activation of regulatory cell-cycle proteins at the oligodendrocytes G1-S checkpoint may constitute a crucial step of the death pathway of oligodendrocytes.
- Subjects :
- CDC25A
Programmed cell death
Ceramide
Cells
Apoptosis
Cell Cycle Proteins
Oligodendrocyte apoptosis
Cell cycle
Ceramides
Biochemistry
Rats, Sprague-Dawley
Proto-Oncogene Proteins c-myc
Cellular and Molecular Neuroscience
chemistry.chemical_compound
c-myc
Proliferating Cell Nuclear Antigen
medicine
PCNA
Animals
cdc25 Phosphatases
Cells, Cultured
cell cycle
oligodendrocyte apoptosis
sphingolipids
Sphingolipids
Cultured
biology
Cell Differentiation
General Medicine
Sphingolipid
Cell biology
Proliferating cell nuclear antigen
Rats
Oligodendroglia
medicine.anatomical_structure
chemistry
biology.protein
Sprague-Dawley
Nucleus
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Neurochemical research 29 (2004): 923–931., info:cnr-pdr/source/autori:Strazza M., Luddi, A.., Brogi A., Ricci M., Santi S., Melli M., Costantino-Ceccarini E./titolo:Activation of cell cycle regulatory proteins in the apoptosis of terminally differentiated oligodendrocytes/doi:/rivista:Neurochemical research/anno:2004/pagina_da:923/pagina_a:931/intervallo_pagine:923–931/volume:29
- Accession number :
- edsair.doi.dedup.....90a5abc74b24f16e4508c338869f3ae4