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Heterologous expression of BetL, a betaine uptake system, enhances the stress tolerance of Lactobacillus salivarius UCC118
- Source :
- Applied and environmental microbiology. 72(3)
- Publication Year :
- 2006
-
Abstract
- Given the increasing commercial and clinical relevance of probiotic cultures, improving the technological robustness of what are often process-sensitive cultures is an important biological goal. The nisin-controlled expression system was used to direct the heterologous expression of the listerial betaine uptake system BetL in the probiotic strain Lactobacillus salivarius UCC118. Following nisin induction, strains expressing betL exhibited a significant increase in resistance to several stresses, including elevated osmo-, cryo-, baro-, and chill tolerance, as well as increased resistance to spray- and freeze-drying. The ability to confer additional stress tolerance on a probiotic culture may be an important step in delivering viable cultures for maximal efficacy.
- Subjects :
- Colony Count, Microbial
Biology
medicine.disease_cause
Applied Microbiology and Biotechnology
Microbiology
law.invention
chemistry.chemical_compound
Probiotic
Betaine
Listeria monocytogenes
Bacterial Proteins
law
Lactobacillus
Freezing
medicine
Nisin
Ecology
Lactobacillus salivarius
Osmolar Concentration
biology.organism_classification
Physiology and Biotechnology
Anti-Bacterial Agents
chemistry
Heterologous expression
Carrier Proteins
Bacteria
Heat-Shock Response
Food Science
Biotechnology
Subjects
Details
- ISSN :
- 00992240
- Volume :
- 72
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Applied and environmental microbiology
- Accession number :
- edsair.doi.dedup.....909de449847954c4035ba3380e2098ff