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CYP71B15 (PAD3) catalyzes the final step in camalexin biosynthesis
- Source :
- Schuhegger, R, Nafisi, M, Mansourova, M, Petersen, B L, Olsen, C E, Svatos, A, Halkier, B A & Glawischnig, E 2006, ' CYP71B15 (PAD3) catalyzes the final step in camalexin biosynthesis ', Plant Physiology, vol. 141, pp. 1248-1254 . https://doi.org/10.1104/pp.106.082024, Plant Physiology
- Publication Year :
- 2006
-
Abstract
- Camalexin represents the main phytoalexin in Arabidopsis (Arabidopsis thaliana). The camalexin-deficient phytoalexin deficient 3 (pad3) mutant has been widely used to assess the biological role of camalexin, although the exact substrate of the cytochrome P450 enzyme 71B15 encoded by PAD3 remained elusive. 2-(Indol-3-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid (dihydrocamalexic acid) was identified as likely intermediate in camalexin biosynthesis downstream of indole-3-acetaldoxime, as it accumulated in leaves of silver nitrate-induced pad3 mutant plants and it complemented the camalexin-deficient phenotype of a cyp79b2/cyp79b3 double-knockout mutant. Recombinant CYP71B15 heterologously expressed in yeast catalyzed the conversion of dihydrocamalexic acid to camalexin with preference of the (S)-enantiomer. Arabidopsis microsomes isolated from leaves of CYP71B15-overexpressing and induced wild-type plants were capable of the same reaction but not microsomes from induced leaves of pad3 mutants. In conclusion, CYP71B15 catalyzes the final step in camalexin biosynthesis.
- Subjects :
- Indoles
Physiology
Recombinant Fusion Proteins
Mutant
Arabidopsis
Plant Science
Saccharomyces cerevisiae
Plant Roots
Catalysis
Mixed Function Oxygenases
chemistry.chemical_compound
Biosynthesis
Cytochrome P-450 Enzyme System
Microsomes
Genetics
Camalexin
Arabidopsis thaliana
Glucuronidase
chemistry.chemical_classification
biology
Arabidopsis Proteins
Phytoalexin
biology.organism_classification
Plants, Genetically Modified
Yeast
Plant Leaves
Thiazoles
Phenotype
chemistry
Biochemistry
Mutation
Microsome
Research Article
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Schuhegger, R, Nafisi, M, Mansourova, M, Petersen, B L, Olsen, C E, Svatos, A, Halkier, B A & Glawischnig, E 2006, ' CYP71B15 (PAD3) catalyzes the final step in camalexin biosynthesis ', Plant Physiology, vol. 141, pp. 1248-1254 . https://doi.org/10.1104/pp.106.082024, Plant Physiology
- Accession number :
- edsair.doi.dedup.....9062ee7d560dc0a7db03a5701b090cba