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Instant super-resolution imaging in live cells and embryos via analog image processing
- Source :
- Nature methods
- Publication Year :
- 2013
-
Abstract
- Existing super-resolution fluorescence microscopes compromise acquisition speed to provide subdiffractive sample information. We report an analog implementation of structured illumination microscopy that enables three-dimensional (3D) super-resolution imaging with a lateral resolution of 145 nm and an axial resolution of 350 nm at acquisition speeds up to 100 Hz. By using optical instead of digital image-processing operations, we removed the need to capture, store and combine multiple camera exposures, increasing data acquisition rates 10- to 100-fold over other super-resolution microscopes and acquiring and displaying super-resolution images in real time. Low excitation intensities allow imaging over hundreds of 2D sections, and combined physical and computational sectioning allow similar depth penetration to spinning-disk confocal microscopy. We demonstrate the capability of our system by imaging fine, rapidly moving structures including motor-driven organelles in human lung fibroblasts and the cytoskeleton of flowing blood cells within developing zebrafish embryos.
- Subjects :
- Fluorescence-lifetime imaging microscopy
Microscope
Materials science
Super-resolution microscopy
business.industry
Image processing
Cell Biology
Analog image processing
Embryo, Mammalian
Biochemistry
Article
law.invention
Data acquisition
Optics
Microscopy, Fluorescence
law
Light sheet fluorescence microscopy
Microscopy
Animals
business
Molecular Biology
Biotechnology
Subjects
Details
- ISSN :
- 15487105
- Volume :
- 10
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Nature methods
- Accession number :
- edsair.doi.dedup.....9062613226ef54825cc0c887c9644824