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A Mucin-Like Protein Is Essential for Oviposition in Nilaparvata lugens
- Source :
- Frontiers in Physiology, Vol 10 (2019)
- Publication Year :
- 2019
- Publisher :
- Frontiers Media SA, 2019.
-
Abstract
- Mucins play a variety of roles; for example, in vertebrates, mucins lubricate epithelial surfaces and protect tissue from physical and biological damage, however, knowledge of insect mucins is limited. Here, we identified an eggshell-related mucin-like protein, NlESMuc, in the brown planthopper (BPH), Nilaparvata lugens. NlESMuc was specifically expressed in the follicular cells from the egg chambers of the ovarioles. RNA interference (RNAi) was used to perform functional analysis of NlESMuc. Adult female BPH with NlESMuc knockdown had significantly reduced fecundity, including more difficult oviposition, lower egg production, and eggs that could not hatch. Scanning electron microscopy showed that, in NlESMuc knocked-down BPH, the ultrastructure of the eggshells of fully developed oocytes was loose, and the cross-section showed many small droplets of about 0.1-μm diameter. Based on the results, it is concluded that NlESMuc is an eggshell-related protein and essential for normal oviposition. Our findings help to provide new targets for pesticide design and RNAi-based BPH control and will also provide new insights into insect eggshells and insect mucins.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Physiology
media_common.quotation_subject
Insect
Biology
01 natural sciences
lcsh:Physiology
03 medical and health sciences
RNA interference
Physiology (medical)
Nilaparvata lugens
Eggshell
media_common
Gene knockdown
lcsh:QP1-981
eggshell
Mucin
RNA
biology.organism_classification
Cell biology
brown planthopper
010602 entomology
mucin-like protein
030104 developmental biology
Ultrastructure
Brown planthopper
oviposition
Subjects
Details
- ISSN :
- 1664042X
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Frontiers in Physiology
- Accession number :
- edsair.doi.dedup.....9060c3078ed25c27967b2f08571c7805