Supplementary Figures 1 through 9 and Supplementary Materials and Methods from Induction of NKG2D Ligands on Solid Tumors Requires Tumor-Specific CD8+ T Cells and Histone Acetyltransferases

Fig. S1. Gradual reduction of Rae-1 during tumor progression. Fig. S2. Induction of Rae-1 by chemotherapy in vitro but not in vivo. Fig. S3 Effector molecule perforin expression in tumors after indicated treatments. Fig. S4 Rae-1 expression is not induced by IL-12 plus doxorubicin in normal tissues. Fig. S5 Accumulation of NKG2D+CD8+ T cells in tumors after IL-12 plus doxorubicin treatment. Fig. S6 Immunochemistry staining of Rae-1 and TUNEL assay in tumor sections. Fig. S7 Rae-1 induction by IL-12 plus doxorubicin only occurs on tumor cells in vivo. Fig. S8 Antitumor cytotoxicity of IL-12 plus doxorubicin abolished by depletion of CD8+ T cells, but not CD4+ T cells or NK cells. Fig. S9 Enrichment of NK cells, CD4+ T cells, or CD8+ T cells in splenocytes collected from BALB/c mice treated with IL-12 plus doxorubicin.