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A novel method to study contraction characteristics of a single cardiac myocyte using carbon fibers
- Source :
- ResearcherID, Scopus-Elsevier
- Publication Year :
- 2001
- Publisher :
- American Physiological Society, 2001.
-
Abstract
- To facilitate cardiac muscle research, we developed a novel method by which the force and length of a single ventricular myocyte can be recorded with a pair of carbon graphite fibers attached firmly to both ends. One fiber was stiff, whereas the other fiber was compliant to allow the recording of force and shortening during twitch contractions. The image of the compliant carbon fiber was projected onto a pair of photodiodes, and their output was fed to a piezoelectric transducer after variable amplifications to alter the effective compliance of the carbon fiber. Thus contraction of the myocyte was induced under virtually isometric conditions as well as under auxotonic conditions. We obtained a bell-shaped relation between the compliance under an auxotonic load and the work output of the myocyte, which was directly related to myocyte performance in the heart. Because it is easy to attach myocytes to the experimental apparatus, the present method would allow us to study cardiac muscle mechanics at the cellular and molecular levels.
- Subjects :
- Male
Cardiotonic Agents
Work output
Materials science
Contraction (grammar)
Physiology
Transducers
Carbon fibers
Cell Separation
Isometric exercise
Isometric Contraction
Physiology (medical)
medicine
Animals
Myocyte
Isotonic Contraction
Ventricular myocytes
Rats, Wistar
Myocardium
Cardiac myocyte
Isoproterenol
Cardiac muscle
Reproducibility of Results
Anatomy
Myocardial Contraction
Rats
medicine.anatomical_structure
visual_art
visual_art.visual_art_medium
Graphite
Cardiology and Cardiovascular Medicine
Microelectrodes
Compliance
Biomedical engineering
Subjects
Details
- ISSN :
- 15221539 and 03636135
- Volume :
- 281
- Database :
- OpenAIRE
- Journal :
- American Journal of Physiology-Heart and Circulatory Physiology
- Accession number :
- edsair.doi.dedup.....905f19019d383cbe2e89d75136427b6f