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Development of Multimarker Diagnostic Models from Metabolomics Analysis for Gestational Diabetes Mellitus (GDM)
- Source :
- Molecularcellular proteomics : MCP. 17(3)
- Publication Year :
- 2017
-
Abstract
- Although metabolomics are desirable to understand the pathophysiology of gestational diabetes mellitus (GDM), comprehensive metabolomic studies of GDM are rare. We aimed to offer a holistic view of metabolites alteration in GDM patients and investigate the possible multimarker models for GDM diagnosis. Biochemical parameters and perinatal data of 131 GDM cases and 138 controls were collected. Fasting serum samples at 75 g oral glucose tolerance test were used for metabolites by ultra performance liquid chromatography-quadrupole-time of flight-mass spectrometry, ultra performance liquid chromatography-triple triple-quadrupole-mass spectrometry and gas chromatography- time-of- flight mass spectrometry platforms. Significant changes were observed in free fatty acids, bile acids, branched chain amino acids, organic acids, lipids and organooxygen compounds between two groups. In receiver operating characteristic (ROC) analysis, different combinations of candidate biomarkers and metabolites in multimarker models achieved satisfactory discriminative abilities for GDM, with the values of area under the curve (AUC) ranging from 0.721 to 0.751. Model consisting of body mass index (BMI), retinol binding protein 4 (RBP4), n-acetylaspartic acid and C16:1 (cis-7) manifested the best discrimination [AUC 0.751 (95% CI: 0.693-0.809), p < 0.001], followed by model consisting of BMI, Cystatin C, acetylaspartic acid and 6,7-diketoLCA [AUC 0.749 (95% CI: 0.691-0.808), p < 0.001]. Metabolites alteration reflected disorders of glucose metabolism, lipid metabolism, amino acid metabolism, bile acid metabolism as well as intestinal flora metabolism in GDM state. Multivariate models combining clinical markers and metabolites have the potential to differentiate GDM subjects from healthy controls.
- Subjects :
- 0301 basic medicine
Adult
medicine.medical_specialty
030209 endocrinology & metabolism
Carbohydrate metabolism
Biochemistry
Models, Biological
Gas Chromatography-Mass Spectrometry
Analytical Chemistry
Bile Acids and Salts
03 medical and health sciences
0302 clinical medicine
Metabolomics
Pregnancy
Internal medicine
Diabetes mellitus
Medicine
Humans
Amino Acids
Molecular Biology
Retinol binding protein 4
biology
business.industry
Research
Area under the curve
Lipid metabolism
medicine.disease
Lipid Metabolism
Gestational diabetes
Diabetes, Gestational
030104 developmental biology
Endocrinology
Glucose
Cystatin C
biology.protein
Female
business
Biomarkers
Chromatography, Liquid
Subjects
Details
- ISSN :
- 15359484
- Volume :
- 17
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Molecularcellular proteomics : MCP
- Accession number :
- edsair.doi.dedup.....904cec44a134657b6dc4e976f59e7b60