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Regulation of SMC traction forces in human aortic thoracic aneurysms
- Source :
- Biomechanics and Modeling in Mechanobiology, Biomechanics and Modeling in Mechanobiology, Springer Verlag, In press, ⟨10.1007/s10237-020-01412-6⟩, Biomechanics and Modeling in Mechanobiology, In press, ⟨10.1007/s10237-020-01412-6⟩
- Publication Year :
- 2021
- Publisher :
- Springer Berlin Heidelberg, 2021.
-
Abstract
- Smooth muscle cells (SMCs) usually express a contractile phenotype in the healthy aorta. However, aortic SMCs have the ability to undergo profound changes in phenotype in response to changes in their extracellular environment, as occurs in ascending thoracic aortic aneurysms (ATAA). Accordingly, there is a pressing need to quantify the mechanobiological effects of these changes at single cell level. To address this need, we applied Traction Force Microscopy (TFM) on 759 cells coming from three primary healthy (AoPrim) human SMC lineages and three primary aneurysmal (AnevPrim) human SMC lineages, from age and gender matched donors. We measured the basal traction forces applied by each of these cells onto compliant hydrogels of different stiffness (4, 8, 12, 25 kPa). Although the range of force generation by SMCs suggested some heterogeneity, we observed that: 1. the traction forces were significantly larger on substrates of larger stiffness; 2. traction forces in AnevPrim were significantly higher than in AoPrim cells. We modelled computationally the dynamic force generation process in SMCs using the motor-clutch model and found that it accounts well for the stiffness-dependent traction forces. The existence of larger traction forces in the AnevPrim SMCs were related to the larger size of cells in these lineages. We conclude that phenotype changes occurring in ATAA, which were previously known to reduce the expression of elongated and contractile SMCs (rendering SMCs less responsive to vasoactive agents), tend also to induce stronger SMCs. Future work aims at understanding the causes of this alteration process in aortic aneurysms. Supplementary information The online version of this article (10.1007/s10237-020-01412-6) contains supplementary material, which is available to authorized users.
- Subjects :
- Male
Cell biomechanics
Mechanotransduction
medicine.medical_treatment
Myocytes, Smooth Muscle
030204 cardiovascular system & hematology
Biology
Traction force microscopy
Models, Biological
03 medical and health sciences
0302 clinical medicine
Vasoactive
medicine.artery
[SPI.MECA.BIOM] Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph]
Extracellular
medicine
Humans
Single cell
Computer Simulation
Process (anatomy)
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
Aged
0303 health sciences
Aorta
Original Paper
Aortic Aneurysm, Thoracic
Mechanical Engineering
[SPI.MECA.BIOM]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph]
Traction (orthopedics)
musculoskeletal system
Phenotype
Smooth muscle cells (smc)
Cell biology
Biomechanical Phenomena
Ascending thoracic aortic aneurysm (ataa)
Modeling and Simulation
Traction force microscopy (tfm)
cardiovascular system
Female
sense organs
Collagen
Biotechnology
Subjects
Details
- Language :
- English
- ISSN :
- 16177940 and 16177959
- Volume :
- 20
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Biomechanics and Modeling in Mechanobiology
- Accession number :
- edsair.doi.dedup.....904248b45d709294cc4a6a26b979206f