Back to Search
Start Over
Genetic analysis of G12P[8] rotaviruses detected in the largest U.S. G12 genotype outbreak on record
- Source :
- Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases. 21
- Publication Year :
- 2013
-
Abstract
- In 2006–07, 77 cases of gastroenteritis in Rochester, NY, USA were associated with rotavirus genotype G12P[8]. Sequence analysis identified a high degree of genetic relatedness among the VP7 and VP4 genes of the Rochester G12P[8] strains and between these strains and currently circulating human G12P[8] strains. Out of 77 samples, two and seven unique nucleotide sequences were identified for VP7 and VP4 genes, respectively. Rochester strain VP7 genes were found to occupy the G12-III lineage and VP4 genes clustered within the P[8]-3 lineage. Six strains contained non-synonymous nucleotide substitutions that produced amino acid changes at 6 sites in the VP8 ∗ region of the VP4 gene. Two sites (amino acids 242 and 246) were located in or near a described trypsin cleavage site. Selection analyses identified one positively selected VP7 site (107) and strong purifying selection at 58 sites within the VP7 gene as well as 2 of the 6 variant sites (79 and 218) in VP4.
- Subjects :
- Microbiology (medical)
Rotavirus
Genotype
Sequence analysis
viruses
Genome, Viral
Biology
medicine.disease_cause
Microbiology
Genetic analysis
Rotavirus Infections
Article
Disease Outbreaks
Evolution, Molecular
Negative selection
fluids and secretions
Phylogenetics
Genetic variation
Genetics
medicine
Humans
Molecular Biology
Gene
Antigens, Viral
Ecology, Evolution, Behavior and Systematics
Phylogeny
Polymorphism, Genetic
Rotavirus Vaccines
virus diseases
Genetic Variation
Virology
United States
Gastroenteritis
Infectious Diseases
Capsid Proteins
Subjects
Details
- ISSN :
- 15677257
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
- Accession number :
- edsair.doi.dedup.....902fa52b8be738039f26aeb715b3d72e