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Plasma Circulating Tumor DNA Levels for the Monitoring of Melanoma Patients: Landscape of Available Technologies and Clinical Applications
- Source :
- BioMed Research International, BioMed Research International, Hindawi Publishing Corporation, 2017, 2017, pp.5986129. ⟨10.1155/2017/5986129⟩, BioMed Research International, 2017, 2017, pp.5986129. ⟨10.1155/2017/5986129⟩, BioMed Research International, Vol 2017 (2017)
- Publication Year :
- 2017
- Publisher :
- HAL CCSD, 2017.
-
Abstract
- Melanoma is a cutaneous cancer with an increasing worldwide prevalence and high mortality due to unresectable or metastatic stages. Mutations inBRAF,NRAS, orKITare present in more than 60% of melanoma cases, but a useful blood-based biomarker for the clinical monitoring of melanoma patients is still lacking. Thus, the analysis of circulating tumor cells (CTCs) and/or cell-free circulating tumor DNA (ctDNA) analysis from blood (liquid biopsies) appears to be a promising noninvasive, repeatable, and systemic sampling tool for detecting and monitoring melanoma. Here, we review the molecular biology-based strategies used for ctDNA quantification in melanoma patients, as well as their main clinical applications. Droplet digital PCR (ddPCR) and next generation sequencing (NGS) technologies appear to be two versatile and complementary strategies to study rare variant mutations for the detection and monitoring of melanoma progression. Among the different clinical uses of ctDNA, we highlight the assessment of molecular heterogeneity and the identification of genetic determinants for targeted therapy as well as the analysis of acquired resistance. Importantly, ctDNA quantification might also be a novel biomarker with a prognostic value for melanoma patients.
- Subjects :
- 0301 basic medicine
Neuroblastoma RAS viral oncogene homolog
MESH: Neoplasm Proteins
Article Subject
[SDV.BBM.BS] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM]
MESH: Melanoma
medicine.medical_treatment
lcsh:Medicine
MESH: DNA, Neoplasm
MESH: Monitoring, Physiologic
Review Article
Bioinformatics
Polymerase Chain Reaction
General Biochemistry, Genetics and Molecular Biology
Targeted therapy
03 medical and health sciences
0302 clinical medicine
Circulating tumor cell
Humans
Medicine
Digital polymerase chain reaction
Neoplasm Metastasis
Melanoma
Monitoring, Physiologic
MESH: Humans
General Immunology and Microbiology
[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM]
business.industry
lcsh:R
High mortality
MESH: Polymerase Chain Reaction
DNA, Neoplasm
General Medicine
medicine.disease
MESH: Neoplasm Metastasis
Neoplasm Proteins
3. Good health
Biomarker
030104 developmental biology
Circulating tumor DNA
030220 oncology & carcinogenesis
Cancer research
business
Subjects
Details
- Language :
- English
- ISSN :
- 23146133 and 23146141
- Database :
- OpenAIRE
- Journal :
- BioMed Research International, BioMed Research International, Hindawi Publishing Corporation, 2017, 2017, pp.5986129. ⟨10.1155/2017/5986129⟩, BioMed Research International, 2017, 2017, pp.5986129. ⟨10.1155/2017/5986129⟩, BioMed Research International, Vol 2017 (2017)
- Accession number :
- edsair.doi.dedup.....901c42c2a274bab209d77eb274776c93
- Full Text :
- https://doi.org/10.1155/2017/5986129⟩