Atom transfer radical polymer-modified paper for improvement in protein fixation in paper-based ELISA

A newly modified paper-based enzyme-linked immunosorbent assay (P-ELISA) was established by immobilizing more proteins on the paper surface through an atom transfer radical polymerization (ATRP) reaction. In addition, introducing graphene oxide (GO) sheets, Au nanoparticles (AuNps) and two primary antibodies (Ab1s) led to signal amplification and cost reduction. Electronic supplementary material The online version of this article (10.1186/s13065-019-0622-7) contains supplementary material, which is available to authorized users.