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Production of Hydroxlated Flavonoids with Cytochrome P450 BM3 Variant F87V and Their Antioxidative Activities
- Source :
- Bioscience, Biotechnology, and Biochemistry. 77:1340-1343
- Publication Year :
- 2013
- Publisher :
- Informa UK Limited, 2013.
-
Abstract
- A variant of P450 BM3 with an F87V substitution [P450 BM3 (F87V)] is a substrate-promiscuous cytochrome P450 monooxygenase. We investigated the bioconversion of various flavonoids (favanones, chalcone, and isoflavone) by using recombinant Escherichia coli cells, which expressed the gene coding for P450 BM3 (F87V), to give their corresponding hydroxylated products. Potent antioxidative activities were observed in some of the products.
- Subjects :
- Chalcone
Bioconversion
Stereochemistry
Recombinant Fusion Proteins
Flavonoid
medicine.disease_cause
Applied Microbiology and Biotechnology
Biochemistry
Antioxidants
Mixed Function Oxygenases
Substrate Specificity
Analytical Chemistry
Structure-Activity Relationship
chemistry.chemical_compound
Bacterial Proteins
Cytochrome P-450 Enzyme System
Escherichia coli
medicine
Molecular Biology
Gene
NADPH-Ferrihemoprotein Reductase
Flavonoids
chemistry.chemical_classification
Recombinant escherichia coli
biology
Organic Chemistry
Cytochrome P450
General Medicine
Monooxygenase
chemistry
biology.protein
Oxidation-Reduction
NADP
Biotechnology
Subjects
Details
- ISSN :
- 13476947 and 09168451
- Volume :
- 77
- Database :
- OpenAIRE
- Journal :
- Bioscience, Biotechnology, and Biochemistry
- Accession number :
- edsair.doi.dedup.....9010d666c6884a5be44aa3fbbd085db5
- Full Text :
- https://doi.org/10.1271/bbb.130148