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Intratumoral T-Cell Infiltrates and MHC Class I Expression in Patients with Stage IV Melanoma
- Source :
- Cancer Research. 65:3937-3941
- Publication Year :
- 2005
- Publisher :
- American Association for Cancer Research (AACR), 2005.
-
Abstract
- The infiltration of tumors by T cells has been shown to correlate with prolonged patients' survival. However, it remains unclear why only some tumors are infiltrated with T cells. This study was designed to investigate possible correlations between intratumoral T-cell infiltrates and the expression of cancer-associated antigens and MHC class I and II molecules in patients with melanoma. Fresh frozen samples from 124 stage IV melanoma patients were analyzed by immunohistochemistry for the expression of Melan-A/MART-1, tyrosinase, gp100, NY-ESO-1, and MHC class I and II. Intratumoral T-cell and B-cell infiltrates were detected by staining with anti-CD4, anti-CD8, anti-CD3, and L26 antibodies. The NY-ESO-1 serum antibody status was assessed by Western blot analysis. Intratumoral CD8+ and CD4+ T cells were detected in 63.9% and 71.3% of patients, respectively. We observed a significant heterogeneity of the expression of the melanocyte differentiation antigens, NY-ESO-1, and MHC class I and II molecules. The only significant correlation was found between the expression of MHC class I and the presence of CD4+ and CD8+ T cells (P < 0.0001). There was a strong association between these two variables with respect to the density and distribution of infiltrating T cells and the pattern of MHC class I expression (focal versus homogenous). Intratumoral T-cell infiltration is closely correlated with the MHC class I expression but not with the expression of differentiation antigens, cancer-associated antigens, or MHC class II molecules. These results may have implications for the definition of prognostic variables and for the identification of patients who may benefit from antigen-specific cancer immunotherapy.
- Subjects :
- CD4-Positive T-Lymphocytes
Cancer Research
Pathology
medicine.medical_specialty
Antibodies, Neoplasm
medicine.medical_treatment
T cell
CD1
CD8-Positive T-Lymphocytes
Lymphocytes, Tumor-Infiltrating
MART-1 Antigen
Melanocyte differentiation
Cancer immunotherapy
Antigen
Antigens, Neoplasm
MHC class I
medicine
Humans
Melanoma
Neoplasm Staging
MHC class II
Membrane Glycoproteins
biology
Monophenol Monooxygenase
Histocompatibility Antigens Class I
Membrane Proteins
Immunohistochemistry
Neoplasm Proteins
medicine.anatomical_structure
Oncology
biology.protein
Cancer research
CD8
gp100 Melanoma Antigen
Subjects
Details
- ISSN :
- 15387445 and 00085472
- Volume :
- 65
- Database :
- OpenAIRE
- Journal :
- Cancer Research
- Accession number :
- edsair.doi.dedup.....8fc5cc7c35abc0ef538725c2cdc5fe8b